We narrowed to 22,610 results for: ESC;

Showing: 9721 - 9740 of 22610 results

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pCAG-G-Flamp1

Plasmid

#188567

Purpose

green biosensor for cAMP in mammalian cells

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1

Tags

His tag

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMALcPP-MS2cp

Plasmid

#203351

Purpose

Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

Depositor

Thomas Cech

Article

Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

Insert

MS2 coat protein

Tags

Hx6-MBP

Expression

Bacterial

Mutation

V75E and A81G, to prevent capsid assembly

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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GPI-RpHLuorin2

Plasmid

#171721

Purpose

GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to GPI anchor

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-mito-EYFP

Plasmid

#174182

Purpose

Express EYFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EYFP with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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ptfLC3

Plasmid

#21074

Purpose

Mammalian expression of rat LC3 fused to mRFP and EGFP

Depositor

Tamotsu Yoshimori

Article

Kimura et al Autophagy. 2007;3(5):452-60.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP and mRFP

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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Lck-mScarlet-I

Plasmid

#98821

Purpose

In vivo visualization of the plasma membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lck

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-DsRed-Express2

Plasmid

#111251

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with DsRed-Express2

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

DsRed-Express2

Expression

Bacterial

Promoter

Pc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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beta-arrestin1 mYFP

Plasmid

#36916

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

beta-arrestin1 (Arrb1 Rat)

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV G-CEPIA1er

Plasmid

#58215

Purpose

Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

G-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

Plasmid

#180263

Purpose

Lentiviral expression vector for dCas9-VP64 in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pZR071_SFFV-dCas9-mCherry-KRAB

Plasmid

#180264

Purpose

Lentiviral expression vector for dCas9-KRAB in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

KRAB and mCherry

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Mito-7

Plasmid

#55102

Purpose

Localization: Mitochondria, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

Insert

Mito (COX8A Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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mEGFP-NbALFA

Plasmid

#159986

Purpose

For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mEGFP-NbALFA

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mEGFP and NbALFA

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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fabp10a:H2B-mGreenLantern

Plasmid

#230045

Purpose

Expression of nuclear green fluorescent protein in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:

Insert

mGreenLantern

Use

Zebrafish expression

Tags

H2B

Promoter

fabp10a

Available Since

Feb. 5, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pC1-SypHer3s

Plasmid

#108118

Purpose

Ratiometric pH probe with enhanced brightness for mammalian cells expression.

Depositor

Vsevolod Belousov

Article

Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

Insert

SypHer3s

Tags

ratiometric YFP

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFEndoNA

Plasmid

#174724

Purpose

Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

Depositor

Jukka Finne

Article

Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

Insert

GFP-endoNA fusion protein

Tags

Histag

Expression

Bacterial

Promoter

T5

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.tgoGAP1

Plasmid

#78737

Purpose

Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

tgoGAP1

Tags

N terminal 81 amino acids of human galactosyltran…

Expression

Mammalian

Mutation

D117A/D119A/D163A in aequorin moiety

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pCAG-GBP1-10gly-Gal4DBD

Plasmid

#49438

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP1-Gal4 DNA binding domain fusion protein

Expression

Mammalian

Mutation

C98S (to increase protein solubility), Q3D

Promoter

CAG

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry3C(1-10)

Plasmid

#117482

Purpose

Lentiviral vector for constitutive expression of sfCherry3C(1-10)

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

sfCherry3C(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

5 substitutions (K45R, G52D, T106A, K182R, N194D)…

Promoter

SFFV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pREDTKI

Plasmid

#51628

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

trc

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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