We narrowed to 66,970 results for: cell;

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pSmart-EF1alpha-AcrIE2-NLS

Plasmid

#207396

Purpose

Expresses AcrIE2 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIE2-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIC9-NLS

Plasmid

#207397

Purpose

Expresses AcrIC9 with NLS in mammalian cells

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIC9-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA ins:R198P mVenus

Plasmid

#210519

Purpose

Expresses mVenus-tagged R198P mutant of Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

Depositor

Jonathan Rocheleau

Article

Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

Insert

Apollo-NADP+

Use

Zebrafish expression, tol2 kit gateway-compatible

Tags

mVenus

Mutation

R198P

Promoter

Zebrafish insulin promoter

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA ins:Apollo-NADP+ mVenus

Plasmid

#210518

Purpose

Expresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

Depositor

Jonathan Rocheleau

Article

Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

Insert

Apollo-NADP+

Use

Zebrafish expression, tol2 kit gateway-compatible

Tags

mVenus

Promoter

Zebrafish insulin promoter

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-p62 var2 PB1mut

Plasmid

#204578

Purpose

Expresses 3xFLAG-p62 var2 PB1mut in mammalian cells

Depositor

Masaaki Komatsu

Article

Kageyama et al Mol Cell Biol. 2018 Jan 16. pii: MCB.006

Insert

p62 (Sqstm1 Mouse)

Expression

Mammalian

Mutation

K7A/D69A

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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MLKP1delKASHec

Plasmid

#130845

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Mutation

Deletion of KASH domain, addition of three alanin…

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKP2del4

Plasmid

#130837

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of terminal four amino acid residues

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKP2delKASH

Plasmid

#130838

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP2

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Deletion of KASH domain, addition of three alani…

Promoter

CaMV 35S

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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MLKP1del4ec

Plasmid

#130844

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

MLKP1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Mutation

Deletion of terminal four amino acid residues

Available Since

Sept. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAV105-PPAB1-GFP-Erk1R84S-1SIM

Plasmid

#190253

Purpose

Expression Erk1-GFP-1SIM in yeast cells

Depositor

Liam Holt

Article

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

ERK1R84S-SIM

Tags

GFP

Expression

Yeast

Mutation

ERK1R84S

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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EK0425 CMV-TO-BFPmut-stop-Bdnf.t+54@202 (FLP-IN)

Plasmid

#191147

Purpose

Expression of the sequence matching the 54 extra bases in EK0362 in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

Bdfn.t+54

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.dora_1

Plasmid

#190700

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 1

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.dora_2

Plasmid

#190701

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 2

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.dora_3

Plasmid

#190702

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 3

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HA-doraWT_rescue_construct

Plasmid

#190608

Purpose

Puromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Pole4 KO sgRNA

Plasmid

#186935

Purpose

Pole4 KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Pole4 KO sgRNA (Pole4 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Pole3 KO sgRNA

Plasmid

#186934

Purpose

Pole3 KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Pole3 KO sgRNA (Pole3 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-SNAP-MDMUT-OCT4

Plasmid

#115692

Purpose

Constitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a non-functional mitotic degron (MDMUT) for control

Depositor

David Suter

Article

Friman et al Elife. 2019 Dec 3;8. pii: 50087. doi: 10

Insert

SNAP-MDMUT-OCT4

Use

Lentiviral

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb-mCherry

Plasmid

#118753

Purpose

Multisite gateway destination vector for 3' tagging with mCherry for cell free expression. Parton lab clone FFF

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

mCherry

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSN526

Plasmid

#184827

Purpose

Expresses human KIF5A(∆exon27) in insect cells

Depositor

Kyoko Chiba

Article

Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

Insert

KIF5A (∆exon27) (KIF5A Human)

Tags

mScarlet-2xStrepII

Expression

Insect

Mutation

∆exon27 form of human KIF5A

Promoter

polyhedrin

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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