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pML323_GOLGA2-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227910

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

GOLGA2 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML494_ATP1B3-C-term_XTEN80-sfGFP-3xHA_pTMPLT

Plasmid

#227912

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

ATP1B3 homology arms with XTEN80-sfGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML499_SLC3A2-C-term_XTEN80-sfGFP-3xHA_pTMPLT

Plasmid

#227913

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SLC3A2 homology arms with XTEN80-sfGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML454_G3BP1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227915

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

G3BP1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML359_CAPRIN1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227916

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CAPRIN1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227891

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

VPS35 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML530_EEA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227892

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

EEA1 homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227900

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

ATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227901

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

MAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML349_PEX3-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227902

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

PEX3 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227904

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227905

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

COPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_YTHDC1_WT

Plasmid

#81799

Purpose

Gateway Donor vector containing YTHDC1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

YTHDC1 (YTHDC1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP13A2 D508N

Plasmid

#171820

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP13A2 D508N

Depositor

Veerle Baekelandt

Article

van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

Insert

human ATP13A2 (ATP13A2 Human)

Use

Lentiviral

Mutation

WT, D962N

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-TP53-2

Plasmid

#121918

Purpose

Encodes sgRNA targeting exon 9 of TP53

Depositor

William Hahn

Article

Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

Insert

TP53 (TP53 Human)

Use

CRISPR

Promoter

U6

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PIK4CA

Plasmid

#20577

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PIK4CA (PI4KA Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
PCDH-METTL14-R298P

Plasmid

#141113

Purpose

Overexpression of METTL14 mutant in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

HMETTL14 (METTL14 Human)

Expression

Mammalian

Mutation

Substitution - Missense, position 298, R➞P

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_FH_WT

Plasmid

#82210

Purpose

Gateway Donor vector containing FH , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FH (FH Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NRAS_WT

Plasmid

#82151

Purpose

Gateway Donor vector containing NRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NRAS (NRAS Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-h-cGASCD(K427E/K428E)

Plasmid

#127162

Purpose

expression of truncated human cGAS protein

Depositor

Thomas Tuschl

Article

Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

Insert

MB21D1 (CGAS Human)

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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