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TET-pLKO-neo CXCR4-sh2

Plasmid

#163742

Purpose

Lentivirus for inducible knockdown of CXCR4 (human)

Depositor

Roland Friedel

Insert

CXCR4 (CXCR4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pUb LwaCas13a + LwaCas13a Guide RNA

Plasmid

#176307

Purpose

Expresses LwaCas13a and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

LwaCas13a

Tags

HA tag

Expression

Insect

Mutation

E656G, I1060V, D1076G- please see depositor comme…

Promoter

Ubi-p63e

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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HA tag NaVSp1 FL pIRES

Plasmid

#128087

Purpose

Mammalian cell expression vector for NaVSp1 bacterial sodium channel. N-terminal HA tag.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Tags

HA

Expression

Mammalian

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-CC2

Plasmid

#51221

Purpose

expression of amino acid 917-1040 of chicken DCTN1 p150Glued in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Expression

Mammalian

Mutation

aa917-1040

Promoter

CMV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Xl-VSP1 C301S

Plasmid

#51882

Purpose

expression of Xl-VSP1 inactive mutant in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 C301S (tpte2 Frog)

Use

Xenopus oocyte expression

Expression

Mammalian

Mutation

C301S, catalytically-inactive mutant of Xl-VSP1

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pTEI166

Plasmid

#126075

Purpose

Vector for expression of mitochondria targeted improved YFP1-10 (T65G, T205S, T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

YFP1-10 (improved)

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI128

Plasmid

#126209

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x3 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11x3 (5 aa linker)

Use

Binary, module 1

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-ADD-L

Plasmid

#127477

Purpose

coding sequence for synthetic zinc finger ZF-ADD-L

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)

Use

Cell-free transcription–translation

Tags

PDZ cognate peptide ligand (VKESLV)

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P4:gs:nls:FKBP

Plasmid

#135984

Purpose

Expresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the FKBP domain

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His:FRB:nls:VPR

Plasmid

#135987

Purpose

Expresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

FRB domain fused to the VPR activation domain

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-RHEB-IRES-Puro

Plasmid

#120576

Purpose

Expresses Flag-RHEB fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-Raf1RBD

Plasmid

#120707

Purpose

Expresses Flag-HA-6xHIS-Raf1 RBD fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Raf1 RBD

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Raf1 amino acids 1-149

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pTRE/SP N184X with 5'UTR

Plasmid

#92375

Purpose

Inducible expression of N184X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

S184X

Promoter

Tet-responsive P tight

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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A337C/Y344C

Plasmid

#24434

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C/Y…

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
Flag‐Tim2

Plasmid

#49213

Purpose

expresses Flag tagged Tim2

Depositor

Lawrence Kane

Article

Knickelbein et al J Immunol. 2006 Oct 15;177(8):4966-70.

Insert

Tim2 (Timd2 Mouse)

Tags

CD8 signal sequence and FLAG

Expression

Mammalian

Mutation

no Tim2 signal sequence; R80L and S196D compared …

Promoter

EF1a

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only
pQTEV-NEUGRIN

Plasmid

#34804

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

NEUGRIN (NGRN Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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K222C/E334C

Plasmid

#24435

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C/E…

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
K420CLM RP

Plasmid

#24443

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S; aa 1-420 only

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pGIPZ EF1a IRES mNeonGreen-P2A-Puro

Plasmid

#248030

Purpose

Backbone that constitutively expresses mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a IRES miRFP720-P2A-Blast

Plasmid

#248029

Purpose

Backbone that constitutively expresses miRFP720 and a blasticidin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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