Addgene

We narrowed to 8,313 results for: PAN

Showing: 2401 - 2450 of 8313 results

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MSCV--Mouse Mdm2 wt-IRES-CFP

Plasmid

#180493

Purpose

Role of Mdm2 splicing variants in tumorigenesis

Depositor

Martine Roussel

Article

Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

Insert

Mdm2 full length (wt)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCVFLAG-ZFP423

Plasmid

#24764

Depositor

Bruce Spiegelman

Article

Gupta et al Nature. 2010 Mar 3. ():.

Insert

Zinc Finger Protein 423 (Zfp423 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2010

Availability

Academic Institutions and Nonprofits only
PMT-mTurquoise2_2

Plasmid

#203776

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mTurquoise2 dimer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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PSICORhp-mp53

Plasmid

#26273

Depositor

Rudolf Jaenisch

Article

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

p53 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
MBP-3C-hNorrin(33-133)-1D4_pAcGP67a

Plasmid

#216384

Purpose

Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218)

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Norrin (NDP Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C and Rho1D4

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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Pdx1 Cre-ER (DM#255)

Plasmid

#15022

Depositor

Douglas Melton

Article

Gu et al Development. 2002 May . 129(10):2447-57.

Insert

Pdx1 promoter (Pdx1 Mouse)

Use

Cre/Lox; Mouse transgenic

Tags

Cre-ER(TM)

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pDWJ27

Plasmid

#207604

Purpose

GFP-BIB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BIB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-PhoCl

Plasmid

#87688

Purpose

Express PhoCl in E. coli

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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BII-ChiNW

Plasmid

#133382

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET11a-STEPtag

Plasmid

#159373

Purpose

Expresses STEPtag in E. coli

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

STEPtag

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB2399

Plasmid

#67550

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked amdSYM marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pH1v1

Plasmid

#60244

Purpose

gRNA expression by the H1 promoter

Depositor

Donald Zack

Article

Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

H1 promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pMAH-Golgi-bapaUGAc

Plasmid

#201675

Purpose

boronic acid- and peptide-assisted UDP-GlcNAc sensor in Golgi

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

Insert

Golgi-bapaUGAc

Use

Tags

Golgi leading sequence

Expression

Mammalian

Mutation

Promoter

CMV SP6

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJS65

Plasmid

#207587

Purpose

GFP-TonB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TonB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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ins-cre_cryaa-Venus

Plasmid

#24333

Depositor

Didier Stainier

Article

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

Cre (cre )

Use

Cre/Lox

Tags

Venus

Expression

Worm

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-GAL4QF

Plasmid

#61309

Purpose

Expresses chimeric transactivator GAL4_DBD:QF2w_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

GAL4_DBD::QF2w_AD

Use

Tags

Expression

Insect

Mutation

Changed EK on C-terminus of QF2 to KKKK

Promoter

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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PMT-mEGFP_2

Plasmid

#203778

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mEGFP dimer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-gLuc-C1C2

Plasmid

#178426

Purpose

Bioluminescence reporter fused to EV membrane binding domain for EV imaging and tracking

Depositor

Masako Harada

Article

Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

Insert

gLuc

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHJ2: LexA DBD

Plasmid

#108240

Purpose

LexA DNA binding domain

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

LexA DNA binding domain (1-87)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO1

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-WT-CGG-R12-KIRV

Plasmid

#63668

Purpose

CGG-R12-KIRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CGG-R12-KIRV RNAP

Use

Tags

Expression

Mutation

Q744K, L747V, N748H, L749I, R756E, L757M, H772R, …

Promoter

T5/Lac

Available since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEntr-SRFflbio

Plasmid

#32971

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

SRF (SRF Human)

Use

Gateway

Tags

flag-bio

Expression

Mutation

Promoter

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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Ngn3 Cre (DM#259)

Plasmid

#15023

Depositor

Douglas Melton

Article

Gu et al Development. 2002 May . 129(10):2447-57.

Insert

Ngn3 promoter (Neurog3 Mouse)

Use

Cre/Lox; Mouse transgenic

Tags

Cre

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
PSICORhp-mp21(v2)

Plasmid

#26272

Depositor

Rudolf Jaenisch

Article

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

p21 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
pAM3558

Plasmid

#40252

Depositor

Susan Golden

Article

Chen et al Plasmid. 2008 May;59(3):176-92. Epub 200

Insert

Gm cassette (BssHII+SphI)

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pJS61

Plasmid

#207585

Purpose

GFP-TolA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TolA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PSICORhp-mp21(v1)

Plasmid

#26271

Depositor

Rudolf Jaenisch

Article

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

p21 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
pQE-WT-CTGA-R13-AKSIRV

Plasmid

#63627

Purpose

CTGA-R13-AKSIRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CTGA-R13-AKSIRV RNAP

Use

Tags

n/a

Expression

Mutation

V725A, Q744K, L747I, N748S, L749I, R756T, Q758K, …

Promoter

T5/Lac

Available since

June 3, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Ngn3 Cre-ER (DM#256)

Plasmid

#15024

Depositor

Douglas Melton

Article

Gu et al Development. 2002 May . 129(10):2447-57.

Insert

Ngn3 promoter (Neurog3 Mouse)

Use

Cre/Lox; Mouse transgenic

Tags

Cre-ER(TM)

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pCfB2375

Plasmid

#67546

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcS-pep1-C1C2

Plasmid

#178428

Purpose

pep1 as cell penetrating peptide fused to EV membrane binging domain (C1C2)

Depositor

Masako Harada

Article

Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

Insert

pep1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL0_11 [pWKS1 ORI]

Plasmid

#198925

Purpose

Level 0 part

Depositor

Cultivarium Tools, Charlie Gilbert, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pWKS1 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
BII-ChBtW

Plasmid

#133368

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
C-12-BE

Plasmid

#135040

Purpose

C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LjCDA1L2_1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPtac

Plasmid

#166937

Purpose

Ptac single-level gene expression controller (SLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Genetic circuit expressing GFP- PTAC

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PTAC

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pE-01

Plasmid

#166930

Purpose

Stop codon part (E)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

STOP CODON TAA and spacer - BLOCK E- Flanked by BsaI sites

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pD-01

Plasmid

#166929

Purpose

TrrnB and L3S2P21 terminator part (D)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

DOUBLE TERMINATOR ( TrrnB - L3S2P21) _BLOCK D - Flanked by BsaI sites

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pB-01

Plasmid

#166924

Purpose

Ptac promoter part (B)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

PTAC promoter - Block B- Flanked by BsaI restriction sites.

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pA-01

Plasmid

#166923

Purpose

LacI expression cassette (A)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

LacI expression cassette - Block A- Flanked by BsaI sites

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2224

Plasmid

#67547

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
N-8-BE

Plasmid

#135023

Purpose

C-T base editing using LpCDA1L1_1 fused to N terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L1_1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE-WT-CTGA-R13-AKSRV

Plasmid

#63622

Purpose

CTGA-R13-AKSRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CTGA-R13-AKSRV RNAP

Use

Tags

Expression

Bacterial

Mutation

V725A, Q744K, L747I, N748S, R756T, Q758K, H772R, …

Promoter

T5/Lac

Available since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
C-1-BE

Plasmid

#135029

Purpose

C-T base editing using PmCDA1L1_4 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

PmCDA1L1_4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
BII-ChPtW

Plasmid

#133379

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
BII-CBtW

Plasmid

#133372

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHJ1_Full length LexA

Plasmid

#107237

Purpose

sfGFP expression repressed by transcriptional factor LexA

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

Full length LexA repressor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLacO1

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
SaMscL (CDelta26)

Plasmid

#79027

Purpose

Staphylococcus aureus MscL with C Terminal Truncation of 26 amino acids

Depositor

Douglas Rees

Article

Liu et al Nature. 2009 Sep 3;461(7260):120-4. doi:

Insert

MscL

Use

Tags

6x Histidine Tag

Expression

Mutation

Stop codon placed before 26 amino acids at C Term…

Promoter

T7 Promoter

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-clbB

Plasmid

#114155

Purpose

Expresses full-length ClbB

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbB

Use

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330.iGFP1

Plasmid

#66581

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pJLI-sup35

Plasmid

#1210

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 5' and 3' flanking regions (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

sup35 gene is not included in this plasmid. Only…

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330.LoxP

Plasmid

#66586

Purpose

sgRNA for LSL

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LoxP sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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