We narrowed to 24,459 results for: Spr

1. lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry

   Plasmid

   #202822

   Purpose

   Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   two sgRNAs that delete exon 1 within the ZNF91 gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 - two

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1a-CRISPRoffv2.1-IRES-BlastR

   Plasmid

   #207180

   Purpose

   Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Insert

   CRISPRoffv2.1
   

   Use

   CRISPR and Lentiviral

   Tags

   HA and TagBFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-PE2-SpRY-P2A-GFP

   Plasmid

   #159981

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpRY-P2A-GFP
   

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

   Plasmid

   #139989

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPR-HOT-P2A-Clover-BlastR

   Plasmid

   #138568

   Purpose

   Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

   Depositor

   Hans Clevers

   Article

   Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

   Insert

   P2A-clover-BlastR
   

   Use

   CRISPR

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-CBE4max-SpRYc-P2A-EGFP

   Plasmid

   #208336

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

   Depositor

   Pranam Chatterjee

   Article

   Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

   Insert

   human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   Sc++ (1-1119), SpRY (1111-1368), D10A

   Promoter

   CAG

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-SpRY-NLS-6xHis (gbr2101)

   Plasmid

   #181743

   Purpose

   bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   bacterial codon optimized SpRY
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS(SV40)-6xHis and MKIEE MBP tag

   Expression

   Bacterial

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   T7

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB-TRE-CRISPRoff-EF1A-TetOn

   Plasmid

   #203355

   Purpose

   Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

   Depositor

   Jonathan Brunger

   Article

   Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

   Insert

   CRISPRoff-TetOn
   

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Px330-EGFP-LRRK2-CRISPR/Cas9

   Plasmid

   #180437

   Purpose

   Plasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   gRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-SFFVp-CRISPRoffv2.1-IRES-BlastR

    Plasmid

    #207181

    Purpose

    Lentiviral Expression Plasmid for CRISPRoff2.1 driven by SFFV Promoter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    CRISPRoffv2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Promoter

    SFFVp

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-multi-CRISPR-sgCasp8_#2-puro

    Plasmid

    #231978

    Purpose

    Knockout mouse Casp8

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-multi-CRISPR-sgCasp8_#1-puro

    Plasmid

    #231977

    Purpose

    Knockout mouse Casp8

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

    Plasmid

    #41863

    Purpose

    This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-IL2sp-6His-Asprosin plasmid

    Plasmid

    #190683

    Purpose

    Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

    Depositor

    Atul Chopra

    Article

    Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

    Insert

    IL2-6xHis-Asprosin
    

    Tags

    IL2 signal peptide, 6xHis

    Expression

    Mammalian

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ6332 - ACTB CRISPRa CLIP Donor

    Plasmid

    #210015

    Purpose

    CLIP donor for integrating CRISPRa (dCas12-miniVPR)-T2A-GFP-P2A to the ACTB locus

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Insert

    dLbCpf1-miniVPR-T2A-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Anti-CASPR/Neurexin IV [K65/35R]

    Plasmid

    #177445

    Purpose

    Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat). Derived from hybridoma K65/35.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant mouse monoclonal antibody (Cntnap1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

    Plasmid

    #70658

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid

    #174137

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

    Plasmid

    #70659

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMD19T-psba1-Ppsba2-dCas9-SpR

    Plasmid

    #73220

    Purpose

    Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

    Depositor

    Paul Hudson

    Article

    Yao et al ACS Synth Biol. 2015 Dec 28.

    Insert

    dCas9 from S. pyogenes
    

    Tags

    c-myc

    Expression

    Bacterial

    Mutation

    Silent mutation at bp 1341 A->C to remove an E…

    Promoter

    PpsbA2

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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