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p5E-QUAS5x

Plasmid

#155113

Purpose

5' entry vector containing QUAS5x promoter element.

Depositor

Bret Pearson

Publication

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUAS5x

Use

Gateway 5' entry vector

Promoter

QUAS5x-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-QUASM1

Plasmid

#155114

Purpose

5' entry vector containing QUASM1 promoter element.

Depositor

Bret Pearson

Publication

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1

Use

Gateway 5' entry vector

Promoter

QUASM1-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

Plasmid

#140198

Purpose

Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.

Depositor

Yit Heng Chooi

Publication

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

dLbCas12a(D156R)-VPR

Use

CRISPR and Synthetic Biology; Expression in asper…

Tags

VPR (VP64-p65-Rta), NLS

Mutation

D832A DNase deactivated, D156R for improved activ…

Promoter

PgpdA

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Venus1-S727A-STAT3

Plasmid

#123174

Purpose

Expresses S727A STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Publication

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

S727A substitution

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Venus2-WTSTAT3

Plasmid

#123165

Purpose

Expresses wild type STAT3 fused to the 158-238 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Publication

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)

Tags

Venus2 (aa 158-238) for bimolecular fluorescence …

Expression

Mammalian

Mutation

none

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRS423-GPD-wrmScarlet1-10-CYC1 terminator

Plasmid

#158584

Purpose

Expresses wrmScarlet1-10 in S. cerevisiae

Depositor

Cynthia Kenyon

Publication

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet1-10

Expression

Yeast

Promoter

GPD

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM38869

Plasmid

#153220

Purpose

Level 0 Arabidopsis U6-promoter vector

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Arabidopsis U6 snRNA promoter

Use

Synthetic Biology

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM37443

Plasmid

#153218

Purpose

Level 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Expression

Plant

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp16

Plasmid

#157724

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp16 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp12

Plasmid

#157710

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp12 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp5-HF

Plasmid

#157687

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp5-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp5

Plasmid

#157686

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp5 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pETM33_Nsp10

Plasmid

#156479

Purpose

Bacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Publication

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp10 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pPSup_iGFP

Plasmid

#149420

Purpose

Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter. The GFP reporter gene harbors the IV2 intron.

Depositor

Stan Fields

Publication

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Type

Empty backbone

Expression

Plant

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223 SARS-CoV-2 NSP5 Cys146Ala

Plasmid

#154028

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP5

Use

Gateway-compatible entry vector, with mutagenized…

Mutation

Many synonymous changes due to codon optimization

Available since

June 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCO602

Plasmid

#139735

Purpose

individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnCPH

Depositor

Daniel Voytas

Publication

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

CPH

Use

Luciferase; Moclo level 0 component

Promoter

none

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP15_nostop

Plasmid

#149317

Purpose

Gateway-compatible Entry vector, with insert of NSP15 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP15 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR223 SARS-CoV-2 NSP14_nostop

Plasmid

#149316

Purpose

Gateway-compatible Entry vector, with insert of NSP14 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP7_nostop

Plasmid

#149310

Purpose

Gateway-compatible Entry vector, with insert of NSP7 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP7 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEBTet-β-tubulin_SNAP

Plasmid

#136831

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Publication

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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