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pSETT4gap

Plasmid

#153413

Purpose

φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

Depositor

Joern Kalinowski

Publication

Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

Type

Empty backbone

Expression

Bacterial

Promoter

gapDH promoter from Eggerthella lenta

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK300

Plasmid

#110592

Purpose

BTK Type 4 terminator for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

rpoC

Use

Synthetic Biology

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pL_FR_Hygro

Plasmid

#127171

Purpose

Frameshift reporter (control plasmid for screening)

Depositor

Paul Blainey

Publication

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

Frameshift Reporter

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1217

Plasmid

#119258

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1071

Plasmid

#119245

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1170

Plasmid

#119252

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCD296.7

Plasmid

#113320

Purpose

dCas9, MCP-TetD, J107 scRNA.b1

Depositor

Jesse Zalatan

Publication

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, MCP-TetD, J107 scRNA.b1_MS2

Use

CRISPR

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMGT1

Plasmid

#109404

Purpose

Tetracycline resistance reporter of ChnR activation

Depositor

Jay Keasling

Publication

Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

Insert

Tetracycline resistance reporter of ChnR activation

Expression

Bacterial

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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UAS-<FRT.STOP<Bxb1

Plasmid

#87643

Purpose

For creating drosophila transgenics expressing UAS-<FRT.STOP<Bxb1

Depositor

Gregory Jefferis

Publication

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Bxb1

Expression

Insect

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBN281

Plasmid

#86726

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Plin-31::FLP D5

Expression

Worm

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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TOPO CMV

Plasmid

#68454

Purpose

TOPO construct expressing CMV for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cytomegalovirus Immediate-Early Promoter

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40

Plasmid

#194690

Purpose

hSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Publication

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40

Plasmid

#194691

Purpose

hSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Publication

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_on-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 2

Plasmid

#206261

Purpose

ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

Depositor

Imre Berger

Publication

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Type

Empty backbone

Use

Multimate/gateway entr 2

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-ALFA

Plasmid

#198301

Purpose

Produces PVCs retargeted with ALFA tag

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-ALFA

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-MoonTag

Plasmid

#198299

Purpose

Produces PVCs retargeted with MoonTag

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-MoonTag

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMN-327

Plasmid

#195452

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF10

Depositor

Ahmad Khalil

Publication

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF10 BS - minTK - mCherry

Use

Lentiviral

Expression

Mammalian

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMN-319

Plasmid

#195447

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF1

Depositor

Ahmad Khalil

Publication

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF1 BS - minCMV - mCherry

Use

Lentiviral

Expression

Mammalian

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMN-320

Plasmid

#195448

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF3

Depositor

Ahmad Khalil

Publication

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF3 BS - minCMV - mCherry

Use

Lentiviral

Expression

Mammalian

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMN-321

Plasmid

#195449

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF10

Depositor

Ahmad Khalil

Publication

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF10 BS - minCMV - mCherry

Use

Lentiviral

Expression

Mammalian

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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