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P3U2C5T2-v1-2 (Pl-lacO1-tetR) (136p)

Plasmid

#70212

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

tetR

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pMaCTag-34

Plasmid

#119998

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-SH3BP4

Plasmid

#46890

Purpose

expresses Myc tagged SH3BP4 in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (SH3BP4 Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pBBK51 pHomL-RPL18Bp-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

Plasmid

#179035

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SCS2tmh, a marker for the endoplasmic reticulum

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-SCS2tmh

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV5-Fyn C3,6S

Plasmid

#19979

Depositor

Marilyn Resh

Article

Alland et al J Biol Chem. 1994 Jun 17. 269(24):16701-

Insert

Fyn (FYN Human)

Expression

Mammalian

Mutation

C3,6S

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only
pRSET FLIPE-1m

Plasmid

#13540

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Bacterial

Mutation

A207W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P01

Plasmid

#120012

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxBFP

Use

Pcr template

Tags

moxBFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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PX552-mScn2a-HaloTag-V5-EF1-sfGFP

Plasmid

#182564

Purpose

For mouse Nav1.2 knockin with HaloTag-V5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn2a gRNA and HaloTag-V5 donor DNA
sfGFP

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE151

Plasmid

#118501

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-E2

Plasmid

#175242

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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