We narrowed to 13,211 results for: CAR

1. pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2]

   Plasmid

   #84445

   Purpose

   AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   Jaws-KGC-GFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   CAG

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLED.GluA2

   Plasmid

   #193017

   Purpose

   GluA2 (Gria2) flip/flop reporter (hSyn promoter, Gria2 flip/flop exons, bichromatic reporter)

   Depositor

   Jonathan Ling

   Article

   Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

   Insert

   Bichromatic reporter (EGFP and dsRed-Express2)
   

   Use

   AAV

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLED.ENS

   Plasmid

   #193016

   Purpose

   Excitatory neuron-specific reporter (hSyn promoter, Synrg exon, bichromatic reporter)

   Depositor

   Jonathan Ling

   Article

   Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

   Insert

   Bichromatic reporter (EGFP and dsRed-Express2)
   

   Use

   AAV

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKan cpRBP-FL P53

   Plasmid

   #174504

   Purpose

   Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)

   Depositor

   Stewart Loh

   Article

   Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do

   Insert

   P53 gene (TP53 Human)
   

   Tags

   20 amino acid linker containing HRV3C protease si…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgTP53(B)

   Plasmid

   #85560

   Purpose

   Expresses sgRNA targeting human TP53 around codon F109

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   tumor protein p53 (TP53 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pET30-2-PHGDH-C234S

   Plasmid

   #107724

   Purpose

   Expression of PHGDH C234S in bacteria

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   PHGDH (PHGDH Human)
   

   Expression

   Bacterial

   Mutation

   C234S

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

   Plasmid

   #128587

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1α (1.1 kb short version)

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM5-C234S PHGDH

   Plasmid

   #83902

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   Phosphoglycerate dehydrogenase (PHGDH Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   quikchanged cysteine 234 to serine

   Promoter

   CMV

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIG-834_NY-ESO-1_TCR_FAS/IL4R

   Plasmid

   #207497

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   FAS/IL4R, NY-ESO-1_TCR (FAS Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEX_305-N-dTAG-FRA1

    Plasmid

    #188742

    Purpose

    expresses murine Fosl1 (Fra1) with N-terminal tag FKBP F36V (dTAG) for the dTAG system

    Depositor

    Günter Schneider

    Article

    Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

    Insert

    Fosl1 (Fosl1 Mouse)
    

    Use

    Lentiviral

    Tags

    FKBP F36V tag (dTAG)

    Expression

    Mammalian

    Promoter

    hPGK promoter

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2AmCherry-W

    Plasmid

    #163176

    Purpose

    Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    WWTR1 (WWTR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT2-shATRX

    Plasmid

    #124258

    Purpose

    Expresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.

    Depositor

    Maria Castro

    Article

    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

    Insert

    shATRX
    

    Expression

    Mammalian

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX304_zeo_mmFbxw7_R505C

    Plasmid

    #160104

    Purpose

    Expresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Fbxw7 alpha R505C (Fbxw7 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    R505C

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.23 C3_7

    Plasmid

    #60293

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    SLC30A8 enhancer (SLC30A8 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-EF1α1.1-Jaws-KGC-tdTomato-ER2

    Plasmid

    #153538

    Purpose

    AAV-mediated expression of Jaws-tdTomato-ER2 under the EF1α promoter (1.1kb short version).

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-tdTomato-ER2
    

    Use

    AAV

    Tags

    ER2, KGC, and tdTomato

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    EF1α1.1

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJM5-mSHMT1

    Plasmid

    #83904

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    Serine hydroxymethyltransferase 1 (Shmt1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-FUS 465-526

    Plasmid

    #44983

    Depositor

    Robin Reed

    Article

    Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

    Insert

    FUS (465-526aa) (FUS Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acids 465-526

    Promoter

    tac

    Available Since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-Syn-FLEX-fd [Chronos-GFP]

    Plasmid

    #84482

    Purpose

    AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    human synapsin promoter

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO_S.AU1.VSVg_NGFR

    Plasmid

    #158351

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.AU1.VSVg

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSN879

    Plasmid

    #190693

    Purpose

    Knockdown mouse KIF5A, KIF5B and KIF5C simultaneously. mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A).

    Depositor

    Shinsuke Niwa

    Article

    Niwa et al Cytoskeleton (Hoboken). 2023 Apr 10. doi

    Insert

    mScarlet::miRNA(KIF5C)::miRNA(KIF5B)::miRNA(KIF5A) (Kif5c, Kif5b, Kif5a Synthetic)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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