159,352 results

1. mCh-Drp1

   Plasmid

   #49152

   Purpose

   mammalian expression of Drp1 fused to mCherry

   Depositor

   Gia Voeltz

   Article

   Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

   Insert

   Drp1 (DNM1L Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

   Plasmid

   #192453

   Purpose

   intronized ttLbCas12a for in planta expression

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
   

   Use

   CRISPR and Synthetic Biology; Moclo compatible le…

   Tags

   Expression

   Mutation

   temperature-tolerant mutation (D156R)

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TFORF3058

   Plasmid

   #144534

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   EBF1 (EBF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

   Plasmid

   #181974

   Purpose

   Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-28z CAR; tNGFR (NGFR Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   NGFR truncation: aa 11-276

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEJS2422_Nme2.Smu-ABE8e-i1

   Plasmid

   #201520

   Purpose

   Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i1

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2.Smu-ABE8e-i1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-mSTING

   Plasmid

   #214156

   Purpose

   Transient expression of STING

   Depositor

   Nan Yan

   Article

   Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

   Insert

   STING (Sting1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. peSpCas9(1.1)-2×sgRNA (empty, donor)

   Plasmid

   #80768

   Purpose

   All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBTK1010 (pSL25)

   Plasmid

   #191005

   Purpose

   Expresses blue chromoprotein on a broad host range RP4 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   blue chromoprotein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CP25

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-BICD2

   Plasmid

   #164626

   Purpose

   Expresses BICD2 in mammalian cells with a GFP tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   BICD2 (BICD2 Human)
   

   Use

   Tags

   Cycle 3 GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET22b_PstS_1

    Plasmid

    #78198

    Purpose

    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A197C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A197C (please see depositors comment below)

    Promoter

    T7

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge