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pTU-B-013

Plasmid

#124416

Purpose

Slot B, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot B + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTU-C-013

Plasmid

#124417

Purpose

Slot C, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot C + terminator

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJK559

Plasmid

#71603

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, tyrosine-…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK562

Plasmid

#71604

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Leu/Trp165>Phe mutant (AaPurE1fwf-Y154L)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, tyrosine-…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK604

Plasmid

#71610

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe mutant (AaPurE1fww-H59N,Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, histidine…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK664

Plasmid

#71613

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-H59N,Y154F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to asparagine, tyrosine-154 …

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK666

Plasmid

#71615

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to asparagine, tryptophan-97…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK563

Plasmid

#71689

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Ile/Trp165>Phe mutant (AaPurE1fwf-Y154I)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, tyrosine-…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK508

Plasmid

#71598

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp165>Phe mutant (AaPurE1fwf-H59N)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes tryptophan-34 to phenylalanine, histidine…

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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Or46a-Gal4

Plasmid

#63179

Purpose

Expresses Gal4 under control of the Drosophila melanogaster Or46a odorant receptor promoter

Depositor

Leslie Vosshall

Article

Fishilevich et al Curr Biol. 2005 Sep 6;15(17):1548-53.

Insert

Or46a promoter

Expression

Insect

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only
pUC119-SA12

Plasmid

#51995

Purpose

Contains the complete genome of the SA12 polymavirus

Depositor

James Pipas

Article

Cantalupo et al J Virol. 2005 Oct;79(20):13094-104.

Insert

SA12

Use

Unspecified

Promoter

none

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_EML4:250-877

Plasmid

#210914

Purpose

Insect expression of human EML4 fragment I250 to A877. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EML4:I250-A877 (EML4 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

K283E natural variant (VAR_031726), I382V Natural…

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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CDH5-AbR-GFP

Plasmid

#122970

Purpose

Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.

Depositor

Budd A. Tucker

Inserts

CDH5p
CopGFP
Zeocin

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pUBQ10-GW

Plasmid

#209370

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by UBQ10 promoter

Depositor

Lucia Strader

Article

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Expression

Plant

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-GluA2-Fab-LC

Plasmid

#230063

Purpose

Expresses light chain Fab fragment of anti-GluA2. Can be used with heavy chain to produce Fab in HEK cells.

Depositor

Matthew Kennedy

Insert

Anti-GluA2 Fab light chain fragment

Expression

Mammalian

Promoter

Chicken beta actin (CAG)

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHIV-Zsgreen

Plasmid

#18121

Depositor

Bryan Welm, Zena Werb

Article

Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

Insert

Zsgreen

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 10, 2010

Availability

Academic Institutions and Nonprofits only

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ampkar

Plasmid

#35097

Purpose

A fluorescent reporter of AMPK activity and cellular energy stress

Depositor

Lewis Cantley

Article

Tsou et al Cell Metab. 2011 Apr 6;13(4):476-86.

Insert

ampkar

Tags

ECFP and Venus

Expression

Bacterial

Mutation

na

Promoter

CMV

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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Ub-G76V-YFP

Plasmid

#11949

Depositor

Nico Dantuma

Article

Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

Insert

Ub-G76V-YFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of YFP. Glycine 76 …

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pET-pro-siRNA-V2

Plasmid

#111086

Purpose

For production of recombinant siRNAs (pro-siRNAs) in E. coli.

Depositor

Linfeng HUANG

Article

Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

Type

Empty backbone

Use

RNAi

Expression

Bacterial

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pet26-MBP-ELIC

Plasmid

#39239

Depositor

Raimund Dutzler

Article

Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

Insert

Eriwinia chrysanthemi - pentameric ligand gated ion channel

Tags

His-10 and MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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