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pTwist-SARS-CoV-2 Spike-deltaC18 JN.1

Plasmid

#216526

Purpose

Encodes SARS-CoV-2 spike for JN.1 pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike JN.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-BFP

Plasmid

#137130

Purpose

Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a DIO-ChRmine-oScarlet-WPRE

Plasmid

#183524

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

ChRmine-oScarlet

Use

AAV

Promoter

Ef1a

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-RNF2

Plasmid

#31216

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

RNF2 (RNF2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

Plasmid

#207805

Purpose

E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.

Depositor

Koen Breyne

Article

Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do

Insert

E-NoMi

Use

Lentiviral

Tags

3xFlag tag, copGFP, mCherry, and nanoluciferase

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR

Plasmid

#171963

Purpose

Donor plasmid for CLTX T-CAR expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

Insert

Synthetic CLTX CD32a-tm CD3z CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-IRES-blast GFP-SPNS1

Plasmid

#198571

Purpose

SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)

Use

Retroviral

Tags

3xFlag-GFP

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-oScarlet

Plasmid

#137136

Purpose

Intersectional viral expression of oScarlet in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-oScarlet

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E95D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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ITGB2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176983

Purpose

Mammalian expression vector encoding ITGB2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

ITGB2 (ITGB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pPtGE34

Plasmid

#107932

Purpose

Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

Depositor

David Edgell

Article

Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

Inserts

OriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3

Use

CRISPR and Synthetic Biology; Episomal vector for…

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Con/Fon-GCaMP6M

Plasmid

#137119

Purpose

Intersectional viral expression of GCaMP6M in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1

Plasmid

#165444

Purpose

Expresses ACX, rXRCC1 in mammalian cells

Depositor

Wei Leong Chew

Article

Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

Insert

APOBEC-nCas9-rXRCC1 (Apobec1 Rat, Synthetic, Streptococcus pyogenes)

Expression

Mammalian

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-hACE2-hygro

Plasmid

#161758

Purpose

Expresses human ACE2 in mammalian cells

Depositor

Neville Sanjana

Article

Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

Insert

Human ACE2 (ACE2 Human)

Use

Lentiviral

Tags

P2A

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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EYFP-p65

Plasmid

#111192

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

RELA (RELA Human)

Tags

EYFP

Expression

Mammalian

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSP1-bio

Plasmid

#47709

Purpose

Expresses enzymatically monobiotinylated full-length MSP1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised MSP1 (MSP1 Plasmodium falciparum, Synthetic)

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pTT3-SP-6xHis-KIR2DL1(FL)-FLAG

Plasmid

#157625

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL1 (KIR2DL1 Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTT3-SP-6xHis-KIR2DL4(FL)-FLAG

Plasmid

#157624

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL4 (KIR2DL4 Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA

Plasmid

#171619

Purpose

AAV floxed Soma-targeted Cal-Light with KA2

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2-msfGFP

Plasmid

#174492

Purpose

bacterial expression of human SEPT2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

His6-TEV and msfGFP

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-E-'No'Mi-Red-T2A-Puro

Plasmid

#246642

Purpose

EF-1alpha driven EV reporter E-'No'Mi-R, a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins. Nanoluc and Flag tag are removable with TEVp.

Depositor

Koen Breyne

Article

Obuchi et al J Extracell Vesicles. 2025 Jun;14(6):e70

Insert

cleavable E-NoMi-Red

Use

Lentiviral

Tags

FLAG-Nanoluciferase and mCherry

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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