Addgene

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pVS1

Plasmid

#33143

Depositor

Michael Rosbash

Article

Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

Insert

LacO (256 copies)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 20, 2011

Availability

Academic Institutions and Nonprofits only
mEGFP-Rac1

Plasmid

#83950

Purpose

Rac1 FLIM donor for dynamic imaging of GTPase activity

Depositor

Ryohei Yasuda

Article

Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyTagMBP

Plasmid

#35050

Depositor

Mark Howarth

Article

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyTagMBP

Use

Tags

His6

Expression

Bacterial

Mutation

Simple peptide tag added to the N-terminus of MBP…

Promoter

T7 promoter

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-split-nLuc

Plasmid

#212612

Purpose

For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-split-nLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

C373T mutation in CVB3

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMBI

Plasmid

#17816

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pAWP78-PVCpnf1-16

Plasmid

#198271

Purpose

Unmodified pPVC; contains WT PVC structural/accessory genes

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf1-16

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE-puro SV40 LT

Plasmid

#13970

Depositor

Thomas Roberts

Article

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

SV40 LT (SV40gp6 )

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha-pcDNA3

Plasmid

#18949

Depositor

William Kaelin

Article

Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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pLVX-dnMCAK

Plasmid

#205995

Purpose

inducible expression of HA-dnMCAK

Depositor

Floris Foijer

Article

Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

Insert

dnMCAK

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

pTight

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-Ruby-9

Plasmid

#197740

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Ruby reporter tricistronic CDS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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