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pCRISPR2_wt

Plasmid

#149386

Purpose

P. aeruginosa PA14 CRISPR2 locus

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_-6D

Plasmid

#149387

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

6bp deleted downstream of the IHFprox site

Promoter

pBAD

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_-5D

Plasmid

#149388

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

5bp deleted downstream of the IHFprox site

Promoter

pBAD

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+5D

Plasmid

#149390

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

5bp inserted downstream of the IHFprox site

Promoter

pBAD

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IHF_Opt

Plasmid

#149395

Purpose

P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

IHFprox site is optimized at key positions needed…

Promoter

pBAD

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IHF_Mut

Plasmid

#149396

Purpose

P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

IHFprox site is mutated at key positions needed f…

Promoter

pBAD

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR2_motif_scram

Plasmid

#149397

Purpose

P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

Upstream motif recognized by Cas1-2/3 during spac…

Promoter

pBAD

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR-PE-bacteria

Plasmid

#172715

Purpose

Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

Cas9n-linker-M-MLV2

Use

Tags

Expression

Bacterial

Mutation

H840A of Cas9

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPRx-Sth1Cas9-L5

Plasmid

#140993

Purpose

Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria

Depositor

Wilbert Bitter

Article

Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

Inserts

Sth1Cas9
TetR
L5 attP
L5 Int
KanR

Use

Unspecified; Integrative in mycobactreia

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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