CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
Type
Blog Post
...pCas9: carries Cas9 and chloramphenicol resistance
pCRISPR: carries a spacer targeting the gene of interest...machinery are first electroporated with pCas9. Then, pCRISPR is introduced along with an oligonucleotide repair...