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pDONR223_KEAP1_p.E611D

Plasmid

#81519

Purpose

Gateway Donor vector containing KEAP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E611D; C624S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.S45F

Plasmid

#81496

Purpose

Gateway Donor vector containing KEAP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S45F; C624S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.G419W

Plasmid

#81505

Purpose

Gateway Donor vector containing KEAP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G419W; C624S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.S102L

Plasmid

#82778

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S102L; C624S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.G603W

Plasmid

#81492

Purpose

Gateway Donor vector containing KEAP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G603W; C624S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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AKAP13

Plasmid

#73230

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

AKAP13 (AKAP13 Human)

Use

Tags

His-6-TEV

Expression

Bacterial

Mutation

pfam00621:RhoGEF 2002-2193, pfam00169:PH 2236-2336

Promoter

T7

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only
CL20_mEGFP_SPAG9_SKAP1

Plasmid

#205925

Purpose

Express mEGFP-tagged fusion protein, SPAG9_SKAP1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

SPAG9_SKAP1 (SPAG9, SKAP1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_RABGAP1L_BCAS3

Plasmid

#205906

Purpose

Express mEGFP-tagged fusion protein, RABGAP1L_BCAS3 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

RABGAP1L_BCAS3 (RABGAP1L, BCAS3 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_NPTN_CLUAP1

Plasmid

#205869

Purpose

Express mEGFP-tagged fusion protein, NPTN_CLUAP1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

NPTN_CLUAP1 (NPTN, CLUAP1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP1-2

Plasmid

#203837

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked out

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1-2

Use

Tags

Expression

Mammalian

Mutation

KO of both AP1 sites

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP1

Plasmid

#203835

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 containing only the downstream AP1 site

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1

Use

Tags

Expression

Mammalian

Mutation

KO of AP1 upstream

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAP14

Plasmid

#186262

Purpose

wgaA (a.k.a expA1) and wgaB (a.k.a expA23), bicistronic, under light light responsive PEL222 promoter and EL222 under constitutively active LacIq promoter

Depositor

Yongku Cho

Article

Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

Inserts

wgaAB bicistronic
EL222 (ELI_04755 Erythrobacter litoralis)

Use

Tags

Expression

Bacterial

Mutation

Promoter

LacIq (CGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCC…

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx4_Rap1

Plasmid

#184662

Purpose

Mammalian expression of mouse Rap1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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MNEAP1ec

Plasmid

#131020

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

MNEAP1

Use

Gateway entry vector

Tags

eGFP-FLAG-HA

Expression

Mutation

Promoter

Available since

May 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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MNEAP1exp

Plasmid

#131021

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

Insert

MNEAP1

Use

Tags

eGFP-FLAG-HA

Expression

Plant

Mutation

Promoter

P35S

Available since

May 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pUAP12008

Plasmid

#177036

Purpose

Level 0 promoter and 5'UTR part for MoClo assembly, GGAG-CCAT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

Cauliflower Mosaic Virus (CaMV) 35S promoter and Tobacco Mosaic Virus (TMV) omega 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_BZRAP1-2/3

Plasmid

#91551

Purpose

Protein expression and purification of human SH3 domain construct BZRAP1-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BZRAP1-2/3 (TSPOAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_BZRAP1-3/3

Plasmid

#91559

Purpose

Protein expression and purification of human SH3 domain construct BZRAP1-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BZRAP1-3/3 (TSPOAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_BZRAP1-1/3

Plasmid

#91487

Purpose

Protein expression and purification of human SH3 domain construct BZRAP1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BZRAP1-1/3 (TSPOAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAP1893-1

Plasmid

#105248

Purpose

human GFP11 with tagRFP 33bp downstream in Lamin A/C (recoded)

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

Insertion of GFP11 at cut tagRFP 33bp downstream (recoded *) in Lamin A/C (LMNA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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