Addgene

We narrowed to 403 results for: RPA

Showing: 81 - 100 of 403 results

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pmiRGLO-RPAIN-202

Plasmid

#117360

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR RPAIN-202

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRPa-iSL(MCS3+4)

Plasmid

#69246

Purpose

Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

Depositor

David Horn

Article

Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

Type

Empty backbone

Use

Expression in trypanosoma brucei

Tags

Expression

Mutation

Promoter

RRNA promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBluescript SK-rPAI-153

Plasmid

#45389

Depositor

Thomas Gelehrter

Article

Zeheb et al Gene. 1988 Dec 20;73(2):459-68.

Insert

Plasminogen activator inhibitor type 1 (Serpine1 Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-hsRPA14 (MSW#503)

Plasmid

#208069

Purpose

Expression of GFP-tagged hsRPA3 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA3 (RPA3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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p23-puro-EGFP-RPA194

Plasmid

#179237

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

RPA194 (POLR1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA70 (MSW#473)

Plasmid

#208067

Purpose

Expression of GFP-tagged hsRPA1 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA1 (RPA1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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p11d-aRPA (MSW#712)

Plasmid

#208078

Purpose

Expression of Alternative human Replication Protein A (RPA1,4,3) in E. coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA1, RPA4, RPA3 (RPA1, RPA3, RPA4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA32 (MSW#497)

Plasmid

#208068

Purpose

Expression of GFP-tagged hsRPA2 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA2 (RPA2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

T88I- please see depositor comments

Promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA (MSW#111)

Plasmid

#208066

Purpose

expression of human Replication Protein A in E. coli

Depositor

Marc Wold

Article

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Insert

RPA1, RPA2, RPA3 (RPA1, RPA3, RPA2 Human)

Use

Tags

Expression

Bacterial

Mutation

RPA32 has a point mutation Thr88(ACC) -> Ile(A…

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-cmlc2-Trpa1b-2A-EGFP

Plasmid

#106405

Purpose

For expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.

Depositor

Randall Peterson

Article

Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

Inserts

cmlc2 promoter
Trpa1b (trpa1b Zebrafish)

Use

Gateway

Tags

2A-EGFP

Expression

Mutation

Promoter

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-ngn1-Trpa1b-2A-mCherry

Plasmid

#106425

Purpose

For expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.

Depositor

Randall Peterson

Article

Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

Inserts

cmlc2 promoter
Trpa1b (trpa1b Zebrafish)

Use

Gateway

Tags

2A-mCherry

Expression

Mutation

Promoter

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only
pEGFP-hsRPA4 (MSW#517)

Plasmid

#208070

Purpose

Expression of GFP-tagged hsRPA4 in human cells

Depositor

Marc Wold

Article

Haring et al Nucleic Acids Res. 2010 Jan;38(3):846-58

Insert

RPA4 (RPA4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pXL-CAG-ICeuI-sHRPaNRX3beta

Plasmid

#79910

Purpose

piggyBac transposon vector, sHRPaNRX3beta

Depositor

Joshua Sanes

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPaNRX3beta

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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Pk_CyRPA-Cd4-bio-His

Plasmid

#126841

Purpose

Expression of recombinant P. knowlesi protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

CyRPA (PKH_052740 Plasmodium knowlesi)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Po_CyRPA-Cd4-bio-His

Plasmid

#126829

Purpose

Expression of recombinant P. ovale protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

CyRPA

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Pm_CyRPA-Cd4-bio-His

Plasmid

#126817

Purpose

Expression of recombinant P. malariae protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

CyRPA (PmUG01_05040800 Plasmodium malariae)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-RPA34-FAPoptim

Plasmid

#221123

Purpose

FAP-tagged marker for the nucleus (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

RPA34-FAPoptim

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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p11d-RPA70/14 (MSW#134)

Plasmid

#208076

Purpose

Expression of human Replication Protein A 70 and 14-kDa subunits in E. coli with 14 having a His-tag

Depositor

Marc Wold

Article

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Insert

RPA1, RPA3 (RPA1, RPA3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF- RPA4 (MSW#716)

Plasmid

#208077

Purpose

Expression of human Replication Protein A 4 in E.coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA4 (RPA4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCA14-pHR-SFFV-GFP-RPA43

Plasmid

#199445

Purpose

Expression of GFP-RPA43 in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

RPA43 (POLR1F Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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