We narrowed to 1,118 results for: gibson assembly

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pWM_12x601_15bpLinker

Plasmid

#157786

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_15bp linker

Expression

Bacterial

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWM_12x601_30bpLinker

Plasmid

#157789

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_30bp linker

Expression

Bacterial

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SspBmicro-mCh-p60

Plasmid

#190168

Purpose

Expresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

Depositor

Anna Akhmanova

Publication

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SspBmicro-mCherry-p60

Tags

SspBmicro-mCherry

Expression

Mammalian

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SpySwitch

Plasmid

#184225

Purpose

Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

Depositor

Mark Howarth

Publication

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

SpySwitch

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pWM_12x601_45bpLinker

Plasmid

#157791

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_45bp linker

Expression

Bacterial

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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EB3N-VVDfast-mCl3-iLID

Plasmid

#190165

Purpose

Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Publication

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EB3N-VVDfast-mClover3-iLID (MAPRE3 Human, Synthetic)

Tags

-VVDfast-mClover3-iLID

Expression

Mammalian

Mutation

1-189 aa from EB3

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Syn-ERT2-iCre-ERT2

Plasmid

#178059

Purpose

Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.

Depositor

Edward Boyden

Publication

Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

ERT2-iCre-ERT2

Use

AAV

Expression

Mammalian

Promoter

Human synapsin 1 (Syn) promoter

Available since

Oct. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAG79-attL1-pegRNA-attL2

Plasmid

#213050

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2

Depositor

Bing Yang

Publication

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_20bpLinker

Plasmid

#157787

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_20bp linker

Expression

Bacterial

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWM_12x601_35bpLinker

Plasmid

#157790

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_35bp linker

Expression

Bacterial

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Halo-Rab6A

Plasmid

#190171

Purpose

Expresses HaloTag-Rab6A in mammalian cells.

Depositor

Anna Akhmanova

Publication

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

HaloTag-Rab6A (RAB6A Human)

Tags

HaloTag

Expression

Mammalian

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EB3N-VVDfast-Halo-iLID

Plasmid

#190166

Purpose

Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Publication

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EB3N-VVDfast-Halo-iLID (MAPRE3 Human, Synthetic)

Tags

-VVDfast-mClover3-iLID

Expression

Mammalian

Mutation

1-189 aa from EB3

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_193 NRL + TetO

Plasmid

#157812

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_tetO

Expression

Bacterial

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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p12x601

Plasmid

#157785

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backbone

Depositor

Michael Rosen

Publication

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

pMD2.G(spei/spei)&12x601_tetO

Expression

Bacterial

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'omega

Plasmid

#223870

Purpose

4G-cloning donor for 3'-Gibson overhang, omega-sequence

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3'omega sequence and spacer

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'alpha

Plasmid

#223863

Purpose

4G-cloning donor for 5'-Gibson overhang, alpha-sequence

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

5'alpha sequence and spacer

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'gamma

Plasmid

#223865

Purpose

4G-cloning donor for 5'-Gibson overhang, gamma-sequence

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

5'gamma sequence and spacer

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'beta

Plasmid

#223864

Purpose

4G-cloning donor for 5'-Gibson overhang, beta-sequence

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

5'beta sequence and spacer

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'delta

Plasmid

#223866

Purpose

4G-cloning donor for 5'-Gibson overhang, delta-sequence

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

5'delta sequence and spacer

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'beta

Plasmid

#223867

Purpose

4G-cloning donor for 3'-Gibson overhang, beta-sequence

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3'beta sequence and spacer

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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