Addgene

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pAAV-TRE3_ mTFP1- Vamp2

Plasmid

#201214

Purpose

AAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminals

Depositor

Tetsuo Yamamori

Article

Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

Insert

mTFP1 (from addgene #54613)

Use

AAV

Tags

mouse Vamp2

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3G

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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LoxP3-Mb2-HA-mTFP1-2A (SO157)

Plasmid

#99615

Purpose

To clone gene of interest downstream of LoxP3-Mb2-HA-mTFP1-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2-HA-mTfp1

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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FRT3-Mb2-HA-mTfp1-2A (IG155)

Plasmid

#99621

Purpose

To clone gene of interest downstream of FRT3-Mb2-HA-mTfp1-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2-HA-mTfp1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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psLIB TRE mTFP1-actin

Plasmid

#229970

Purpose

encodes a mTFP1-actin fusion construct under the control of a dox inducible promoter

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

mTFP1-actin

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFOX14-Tier1-PhCMV-mTFP1

Plasmid

#169511

Purpose

Tier-1 vector encoding PhCMV-driven green fluorescent protein mTFP1 (PhCMV-mTFP1-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven monomeric cyan fluorescent protein mTFP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 2 CMV mTFP1 Actin

Plasmid

#206255

Purpose

ENTR Vector 2 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTFP1 Actin under the control of CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mTFP1 Actin

Use

Multimate/gateway entr 2

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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C232-E08: attB2r-mTFP1-attB3

Plasmid

#162904

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-3xFLAG-CMVp-mito-mTFP1

Plasmid

#182376

Purpose

Dual expression construct encoding 3xFLAG and mito-mTFP1 from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

3xFlag
mito-mTFP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter and SV40 promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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C512-E11: attB5r-mTFP1-nostop-attB1r

Plasmid

#162942

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-mTFP1.0-optoNES-WPRE

Plasmid

#159945

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-mTFP1.0-optoNES

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

Plasmid

#101299

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Use

Tags

Expression

Mammalian

Mutation

Contains amino acids 1-698 and 698-906 of alpha-C…

Promoter

Available since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

Plasmid

#101297

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Use

Tags

Expression

Mammalian

Mutation

Contains amino acids 1-906 of alpha-Catenin

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)

Plasmid

#101298

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Use

Tags

Expression

Mammalian

Mutation

Contains amino acids 1-698 of alpha-Catenin

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1

Plasmid

#182380

Purpose

Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

mtIF3 (Mtif3 Mouse)
mito-mTFP1

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Changed CDS sequence from 'ccacgttcaagtcacga…

Promoter

CMV promoter and SV40 promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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AiP12700 - pAAV-AiE0452h-minBG-mTFP1-WPRE3-BGHpA (Alias: CN2700)

Plasmid

#191708

Purpose

AI plasmid ALIAS: AiP12700. Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

mTFP1

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12965 - pAAV-AiE0444h-minBG-mTFP1-WPRE3-BGHpA (Alias: CN2965)

Plasmid

#191729

Purpose

AI plasmid ALIAS: AiP12965. Expression of mTFP1 (monomeric teal fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs), enrichment in dorsal striatum over ventral striatum

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

mTFP1

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12542: pAAV-AiE0410m-minBG-mTFP1-WPRE3-BGHpA (Alias: CN2542)

Plasmid

#236603

Purpose

AiE0410m is an enhancer sequence, designed to drive AAV-mediated transgene expression in oligodendrocytes

Depositor

Jonathan Ting

Article

Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.

Insert

mTFP1

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14530: pAAV-AiE0743m_3xC2-minBG-mTFP1-WPRE3-BGHpA (Alias: CN4530)

Plasmid

#230869

Purpose

AiE0743m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic interneurons

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.

Insert

mTFP1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14531: pAAV-hDLX_C2.1-minBG-mTFP1-WPRE3-BGHpA (Alias: CN4531)

Plasmid

#230870

Purpose

hDLX_C2.1 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic interneurons

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.

Insert

mTFP1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13808 - pAAV-AiE0140h_3xC2-minBG-mTFP1-WPRE3-BGHpA (Alias: CN3808)

Plasmid

#224176

Purpose

AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+ neurons (note cortical Pvalb+ and L5_ET)

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

mTFP1

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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