We narrowed to 596 results for: pFAST

1. pAG668 lifeAct-pFAST

   Plasmid

   #172866

   Purpose

   Expresses LifeAct-pFAST in mammalian cells (actin labeling)

   Depositor

   Arnaud Gautier

   Article

   Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

   Insert

   LifeAct-pFAST
   

   Tags

   Myc tag (C terminal on insert)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac1-LRRK2 WD40

   Plasmid

   #139211

   Purpose

   Human LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.

   Depositor

   Hao Wu

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2019 Jan 29;11

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   N-His and MBP

   Expression

   Insect

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac1_GI.1_N116C-G193C

   Plasmid

   #162580

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Asparagine 116 was mutated to Cysteine and Glycin…

   Promoter

   polyhedrin

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1/MBP-His

   Plasmid

   #38008

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   MBP (Maltose binding protein)
   

   Use

   Baculovirus expression

   Tags

   6xHIS

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pFastBacI-KDAC8 H143A

   Plasmid

   #224344

   Purpose

   Expresses human KDAC8 (HDAC8) H143A in insect cells

   Depositor

   Terry Watt

   Article

   Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

   Insert

   KDAC8 (HDAC8 Human)
   

   Tags

   TEV-cleavable His6

   Expression

   Insect

   Mutation

   Histidine 143 mutated to alanine

   Promoter

   Polyhedrin

   Available Since

   Oct. 22, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pFastBacI-KDAC8 D101E

   Plasmid

   #224350

   Purpose

   Expresses human KDAC8 (HDAC8) D101E in insect cells

   Depositor

   Terry Watt

   Article

   Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

   Insert

   KDAC8 (HDAC8 Human)
   

   Tags

   TEV-cleavable His6

   Expression

   Insect

   Mutation

   Aspartate 101 mutated to glutamate

   Promoter

   Polyhedrin

   Available Since

   Oct. 22, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pFastBacDual with LFn + Needle

   Plasmid

   #84868

   Purpose

   Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   Needle
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG671 mito-pFAST

   Plasmid

   #172867

   Purpose

   Expresses mitochondrial targeting domain-pFAST in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

   Insert

   mito-pFAST
   

   Tags

   Myc tag (C terminal on insert)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG657 H2B-pFAST

   Plasmid

   #172862

   Purpose

   Expresses pFAST fused to zebrafish histone H2B in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

   Insert

   H2B-pFAST
   

   Tags

   Myc tag (C terminal on insert)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBacDual with LFn + Fla

    Plasmid

    #84866

    Purpose

    Baculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    Fla
    

    Use

    Baculovirus

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFastBac1_GI.1_Q62C-A140C

    Plasmid

    #162581

    Purpose

    Expresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cells

    Depositor

    Peter Kwong

    Article

    Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

    Insert

    VP1 protein from human norovirus GI.1
    

    Expression

    Insect

    Mutation

    Glutamine 62 changed to Cysteine and Alanine 140 …

    Promoter

    polyhedring

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastbac-Flag-BRCA1

    Plasmid

    #223228

    Purpose

    Insect expression construct for Flag-tagged BRCA1 protein production and purification

    Depositor

    Weixing Zhao

    Article

    Wang et al Mol Cell. 2023 Oct 19;83(20):3679-3691.e

    Insert

    Breast Cancer 1 (BRCA1 Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBac HTB DPP9L

    Plasmid

    #166788

    Purpose

    Insect cell expression vector for DPP9L (protein purification)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Tags

    HIS-TEV

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac HTB DPP9S

    Plasmid

    #166787

    Purpose

    Insect cell expression vector for DPP9S (protein purification)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Tags

    HIS-TEV

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBac-His6-Mps1-WT

    Plasmid

    #114063

    Purpose

    protein production

    Depositor

    Geert Kops

    Article

    Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

    Insert

    Mps1-WT (TTK Human)
    

    Tags

    His6

    Expression

    Insect

    Mutation

    none

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG665 MAP4-pFAST

    Plasmid

    #172865

    Purpose

    Expresses MAP4-pFAST in mammalian cells (microtubule labeling)

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    MAP4-pFAST
    

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastBac flag Bmi1

    Plasmid

    #1967

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    Bmi1 (Bmi1 Mouse)
    

    Tags

    flag

    Expression

    Insect

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pFastBac GST-SAS6

    Plasmid

    #80273

    Purpose

    baculovirus creation plasmid for GST-SAS6

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    SAS6 (SASS6 Human)
    

    Tags

    GST

    Expression

    Insect

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac K-Flag Ring1A

    Plasmid

    #1995

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    Ring1A (Ring1 Mouse)
    

    Tags

    flag

    Expression

    Insect

    Mutation

    Kozak before start codon

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pFastBac GST-Plk4

    Plasmid

    #80264

    Purpose

    baculovirus creation plasmid for GST-Plk4

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Tags

    GST

    Expression

    Insect

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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