Skip to main content
Addgene
Showing: 81 - 100 of 1591 results

  1. pC1300_pUB10_pcoCASphi_E9t_V2_2x35Sp_HSP18t_ribozyme_AtPDS3_gRNA10

    Plasmid
    #197959
    Purpose
    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by 2x35Sp and flanked by ribozymes.
    Depositor
    Steven Jacobsen
    Article
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Inserts
    pcoCasphi-2
    AtPDS3 gRNA10
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S promoter and pUBQ10
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197959-plasmid-map-sequence-id-387883

    Enlarge

  5. pMSP1D12W/2Y

    Plasmid
    #124275
    Purpose
    Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence
    Depositor
    Alexey S. Ladokhin
    Insert
    Membrane scaffold protein
    Use
    Tags
    7xHis-tag
    Expression
    Bacterial
    Mutation
    Two tryptophans changed to two tyrosines
    Promoter
    T7
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    124275-plasmid-map-sequence-id-242402

    Enlarge

  8. pN3-Sp1FL

    Plasmid
    #24543
    Depositor
    Guntram Suske
    Insert
    Sp1FL (SP1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    24543-plasmid-map-sequence-id-209002

    Enlarge

  13. MSP1-bio

    Plasmid
    #47709
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP1 (MSP1 Synthetic, Plasmodium falciparum)
    Use
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47709-plasmid-map-sequence-id-68315

    Enlarge

  16. pUD-MSP1D1

    Plasmid
    #161608
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    UD-MSP1D1
    Use
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1; all tryptophans and tyrosines w…
    Promoter
    T7
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161608-plasmid-map-sequence-id-317655

    Enlarge

  17. pMSP12::mCherry

    Plasmid
    #30169
    Depositor
    Lalita Ramakrishnan
    Insert
    Mycobacterium Strong Promoter (MSP)
    Use
    Mycobacteria expression
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2011
    Availability
    Academic Institutions and Nonprofits only
    30169-plasmid-map-sequence-id-214699

    Enlarge

Showing: 81 - 100 of 1591 results