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pRS DV DNM2-mRUBY2

Plasmid

#107793

Purpose

donor vector for knock-in gene editing at human DNM2 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

DNM2-mRuby2 (DNM2 Human, Synthetic)

Use

Yeast-e.coli shuttle vector

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGK-Ptrf-mKate2

Plasmid

#128765

Purpose

Fluorescent mKate reporter for Ptrf

Depositor

Deepak Srivastava

Article

Stone et al Cell Stem Cell. 2019 Jul 3;25(1):87-102.

Insert

Ptrf promoter (Cavin1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ptrf

Available since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-EF1A>Tet3G:IRES:Neo

Plasmid

#198756

Purpose

Vector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Tet3G, IRES, Neo

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1A

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a-L274GMmMetRS-T2A-mCherry

Plasmid

#220800

Purpose

Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

Depositor

Elizabeth Kirby

Article

Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

Insert

Mars1 (Mars1 Mouse)

Use

Lentiviral

Tags

2xFLAG and T2A-mCherry

Expression

Mutation

mutation in MetRS to change aa 274 from L to G

Promoter

EF1a

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGK-Ldb3-mKate2

Plasmid

#128766

Purpose

Fluorescent mKate reporter for Ldb3

Depositor

Deepak Srivastava

Article

Stone et al Cell Stem Cell. 2019 Jul 3;25(1):87-102.

Insert

Ldb3 promoter (Ldb3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ldb3

Available since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLV[TetOn]-Puro-TRE3G>mAscl1

Plasmid

#198755

Purpose

Vector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLV[TetOn]-Puro-TRE3G>mNeurog2

Plasmid

#198754

Purpose

Vector encodes the gene for murine neurogenin-2 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Neurogenin-2 (Neurog2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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8061-E24

Plasmid

#234261

Purpose

Gateway ORF Entry clone of human AMHR2 with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

AMHR2 (AMHR2 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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spCas9-BlastR (pCBhCas9-BlastR)

Plasmid

#71489

Purpose

Expresses SpCas9 and Blasticidin resistance

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Insert

spCas9

Use

Tags

Expression

Mutation

Promoter

CBh

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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sgPal7 (p2Tol-U6-sgPal7-HygR)

Plasmid

#71484

Purpose

Expresses palindromic sgRNA for use in self-cloning CRISPR

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Insert

sgPal7

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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sgPal1 (p2Tol-U6-sgPal1-HygR)

Plasmid

#71483

Purpose

Expresses palindromic sgRNA for use in self-cloning CRISPR

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Insert

sgPal1

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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Bneu-9

Plasmid

#205214

Purpose

Unit Bactoneuron-9, expresses EGFP

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMJH46

Plasmid

#67272

Purpose

Conjugally transferable red recombinogenic plasmid

Depositor

Mark Liles

Article

Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

Insert

traJ, cat, oriT

Use

Red recombinase

Tags

Expression

Mutation

Promoter

cat gene promoter from PGNS-BAC

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Orai3

Plasmid

#16370

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai3 (ORAI3 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

Plasmid

#71485

Purpose

Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pRVGP

Plasmid

#15377

Depositor

Stephen Smale

Article

Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

Insert

d2EGFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2008

Availability

Academic Institutions and Nonprofits only
pQsupR

Plasmid

#15378

Depositor

Stephen Smale

Article

Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

Insert

human H1 promoter

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2008

Availability

Academic Institutions and Nonprofits only
Bneu-10

Plasmid

#205215

Purpose

Unit Bactoneuron-10, expresses E2-crimson

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Inserts

E2-Crimson
LuxR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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P2X4-pHluorin123

Plasmid

#52926

Purpose

express P2X4 protein with pHluorin at 123rd position

Depositor

Baljit Khakh

Article

Xu et al J Gen Physiol. 2014 Jul;144(1):81-104. d

Insert

mP2X4-pHluorin123

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only
pFLAG_OriM

Plasmid

#110097

Purpose

Analogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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