Showing: 81 - 100 of 107 results
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Plasmid#138537PurposeFor making Beta-catenin-S33Y RNADepositorInsertBeta-catenin
UseIn vitro transcriptionTags2XFLAG and 2xNLSExpressionMutationchanged serine 33 to tyrosinePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
HA-USPL1-C236S-pcDNA3.1
Plasmid#85763Purposemammalian expression of full length HA tagged USPL1, catalytic inactive variant C236SDepositorInsertUSPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
UseTagsHAExpressionMammalianMutationcatalytic inactive mutant C236SPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
HA-USPL1-C236A-pcDNA3.1
Plasmid#85764Purposemammalian expression of full length HA tagged USPL1, catalytic inactive variant C236ADepositorInsertUSPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
UseTagsHAExpressionMammalianMutationcatalytic inactive mutant C236APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
USPL1cat-pEYFP_C1
Plasmid#85766Purposemammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusionDepositorInsertUSPL1, catalytic domain (aa 212-514) (USPL1 Human)
UseTagsEYFPExpressionMammalianMutationcatalytic domain (aa 212-514)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
USPL1cat-pETM30
Plasmid#85768Purposebacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusionDepositorInsertUSPL1, catalytic domain (aa 212-514) (USPL1 Human)
UseTagsGST and HisExpressionBacterialMutationcatalytic domain (aa 212-514)PromoterT7-lacAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168
Plasmid#133976Purposemammalian expression vector of Flag tagged wild type RNF168DepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-eGFP-NLS-RNF168
Plasmid#133977Purposemammalian expression vector of eGFP tagged wild type RNF168DepositorInsertRNF168 (RNF168 Human)
UseTagseGFPExpressionMammalianMutationsiRNA resistant sequence: GAGGAGTCGTGTTTATTGAPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCW57-mCherry-2A-BRD4 Iso CdelET
Plasmid#137723PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)DepositorInsertBRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationDeleted amino acids 600-682PromoterTight TRE promoterAvailabilityAcademic Institutions and Nonprofits only -
USPL1cat-C236S-pEYFP_C1
Plasmid#85767Purposemammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion, catalytic inactive variant C236SDepositorInsertUSPL1, catalytic domain (aa 212-514), catalytic inactive variant C236S (USPL1 Human)
UseTagsEYFPExpressionMammalianMutationcatalytic domain (aa 212-514), catalytic inactiv…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168 delta MIU1
Plasmid#133978Purposemammalian expression vector of Flag tagged RNF168 where the first motif interacting with ubiquitin (MIU) has been deletedDepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of MIU1PromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-Flag-RNF168 delta MIU2
Plasmid#133979Purposemammalian expression vector of Flag tagged RNF168 where the second motif interacting with ubiquitin (MIU) has been deletedDepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of MIU2PromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-Flag-RNF168 delta RING
Plasmid#133982Purposemammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)DepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of RINGPromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pCW57-mCherry-2A-BRD4 Iso AdelCTD
Plasmid#137722PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)DepositorInsertBRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationTruncated at amino acid 1328PromoterTight TRE promoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-eGFP-FRT-T0-RNF168 delta MIU1/MIU2
Plasmid#133980Purposeinducible mammalian expression vector of eGFP tagged RNF168 where both motifs interacting with ubiquitin have been deletedDepositorInsertRNF168 (RNF168 Human)
UseTagseGFPExpressionMammalianMutationdeletion of MIU1 and MIU2PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF168 delta MIU1/MIU2
Plasmid#133981Purposemammalian expression vector of Flag tagged RNF168 where both motifs interacting with ubiquitin have been deletedDepositorInsertRNF168 (RNF168 Human)
UseTagsFlagExpressionMammalianMutationdeletion of MIU1 and MIU2PromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-eGFP-NLS-RNF168 delta RING
Plasmid#133983Purposemammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)DepositorInsertRNF168 (RNF168 Human)
UseTagseGFPExpressionMammalianMutationdeletion of RING; siRNA resistant sequence: GAGGA…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
lucMAPT-30D
Plasmid#214672PurposeMAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice siteDepositorInsertMAPT (MAPT Human)
UseTagsFirefly Luciferase, Renilla Luciferase, and V5ExpressionMammalianMutationContains Exon 9, Exon 10 with a mutation to intro…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
lucMAPT-30U
Plasmid#214673PurposeMAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice siteDepositorInsertMAPT (MAPT Human)
UseTagsFirefly Luciferase, Renilla Luciferase, and V5ExpressionMammalianMutationContains Exon 9, Exon 10 with a mutation to intro…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
mGFP-hNET
Plasmid#193364Purposeexpression of human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cellsDepositorInserthuman Norepinephrine Transporter (SLC6A2 Human)
UseTagsmonomeric GFP (mGFP)ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
mGFP-hNET-A457P
Plasmid#193365Purposeexpression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cellsDepositorInserthuman Norepinephrine Transporter (A457P mutation) (SLC6A2 Human)
UseTagsmonomeric GFP (mGFP)ExpressionMammalianMutationA457P (GCC to CCC)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 81 - 100 of 107 results