We narrowed to 2,368 results for: pcas9

1. pFH54

   Plasmid

   #128183

   Purpose

   Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFH55

   Plasmid

   #125814

   Purpose

   Level0 nSpCas9-PmCDA, Arabidopsis codon optimized

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   nSpCas9-PmCDA, Arabidopsis codon optimized
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSL1045

   Plasmid

   #129525

   Purpose

   pDEF-SpCas9, Empty Vector for CRISPRi, PbEF1a promoter, 1st Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   dSpCas9 (Other)
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFH24

   Plasmid

   #131763

   Purpose

   Level0 SpCas9, wheat codon optimized (version 2)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   SpCas9, wheat codon optimized, version 2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFH23

   Plasmid

   #128180

   Purpose

   Level1 ZmUBIp::SpCas9 (wheat codon optimized):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::SpCas9 (wheat codon optimized):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFH53

   Plasmid

   #128182

   Purpose

   Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFH52

   Plasmid

   #128181

   Purpose

   Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFH68

   Plasmid

   #128188

   Purpose

   Level1 ZmUBIp::SpCas9-NG (human codon optimized):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::SpCas9-NG (human codon optimized):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LentiCRISPR v2 sgZER1-1

   Plasmid

   #191549

   Purpose

   Expression of spCas9 and sgRNA targetting ZER1

   Depositor

   Elizabeth White

   Article

   Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

   Insert

   spCas9 (ZER1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LentiCRISPR v2 sgZER1-2

    Plasmid

    #191550

    Purpose

    Expression of spCas9 and sgRNA targetting ZER1

    Depositor

    Elizabeth White

    Article

    Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

    Insert

    spCas9 (ZER1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFH25

    Plasmid

    #131764

    Purpose

    Level0 SpCas9, wheat codon optimized (version 3)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    SpCas9, wheat codon optimized, version 3
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330S-2

    Plasmid

    #58778

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRDA_479

    Plasmid

    #179099

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    ABE8e (SpG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRDA_426

    Plasmid

    #179097

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    ABE8e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330S-3

    Plasmid

    #58779

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYPQAT01

    Plasmid

    #223373

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRDA_336

    Plasmid

    #179095

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    BE3.9 (Cas9-NG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRDA_478

    Plasmid

    #179096

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    BE3.9 (SpG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQAT05

    Plasmid

    #223377

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge