We narrowed to 16,606 results for: Emb

1. FB030

   Plasmid

   #119714

   Purpose

   Terminator paf from P. chrysogenum domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   Terminator paf
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CD8a-f-CTS-EGFP

   Plasmid

   #86063

   Purpose

   A mammalian expression plasmid encoding fibrocystin mimentic clone CD8a-f-CTS (CD8a luminal and transmembrane domains, fibrocystin ciliary targeting signal domains) with C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Fibrocystin (Pkhd1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains CD8a luminal and transmembrane domains a…

   Promoter

   CMV

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FB005

   Plasmid

   #119678

   Purpose

   Coding sequence nptII from E. coli domesticated into pUPD2 with AATG/GCTT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   geneticin resistance marker
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FB002

   Plasmid

   #119676

   Purpose

   Terminator tub from Neurospora crassa domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   Terminator tub
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FB008

   Plasmid

   #119706

   Purpose

   Terminator trpC from Aspergillus nidulans domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   Terminator trpC
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FB001

   Plasmid

   #119057

   Purpose

   Promoter trpC from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   Promoter trpC
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA3_altPKA-Cα-mPAGFP

   Plasmid

   #114133

   Purpose

   Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   Testis only splice variant of mouse PKA catalytic subunit α that has no myristoylation site, C-terminally tagged by mPAGFP (Prkaca Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3_nmPKA-Cα-mPAGFP

   Plasmid

   #114131

   Purpose

   Mammalian expression of the G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with G2A mutation (Prkaca Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

   Plasmid

   #224512

   Purpose

   A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   3xHA-BirA-T2A-mCherry-Caax
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Dyn1 R271E D291R

    Plasmid

    #198342

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R271E D291R

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Dyn1 R271E

    Plasmid

    #198343

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R271E

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Dyn1 R271E E720R

    Plasmid

    #198344

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R271E D291R

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Dyn1 K44A

    Plasmid

    #198340

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Dyn1 D406A

    Plasmid

    #198341

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406A

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15

    Plasmid

    #207054

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    L55W T56A V61W R65A

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pS238D1::sptse5-CT

    Plasmid

    #192959

    Purpose

    For expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studies

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    sptse5-CT
    

    Use

    Tags

    PelB signal peptide

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1317)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-Hygro-EF1A-RG-Linker-eGFP

    Plasmid

    #176071

    Purpose

    EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    RG
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCRISPRv2-POLB-KO-g1

    Plasmid

    #176090

    Purpose

    Cas9 plus POLB gRNA #1; contains a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    POLB sgRNA #1 (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-Miro1 (412-618)

    Plasmid

    #127628

    Purpose

    GTPase2 domain of human Miro1 including transmembrane domain and C-terminal amino acids

    Depositor

    Josef Kittler

    Article

    Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

    Insert

    Miro1 (RHOT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    412-618 only

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3_PKA-Cα(S11D)-mPAGFP

    Plasmid

    #114135

    Purpose

    Mammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge