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Plasmid#158494PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 D481V/L17E without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationL17E, D481V, no STOP codonPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA2
Plasmid#158480PurposeEntry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 D489V with STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationD489V, STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA11
Plasmid#158489PurposeEntry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 L17E without STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationL17E no STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA9
Plasmid#158487PurposeEntry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationno STOP codonPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA4
Plasmid#158482PurposeEntry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E/D489V with STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationL17E, D489V, STOP codonPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA10
Plasmid#158488PurposeEntry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V without STOP codonDepositorInsertZAR1 (ZAR1 Mustard Weed)
UseGolden gate entry vectorTagsExpressionMutationD489V, no STOP codonPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3
Plasmid#189752PurposeProduces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysisDepositorInsertmTurquoise2_spatial-linker-P2A_mScarlet3
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV2_CAG_oROS-G
Plasmid#216115PurposeEncodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.DepositorInsertoROS-G
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet-I3-NCwt-SYFP2
Plasmid#189765PurposeDual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet-I3-NCwt-SYFP2
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3
Plasmid#189755PurposeProduces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysisDepositorInsertmTurquoise2_spatial-linker-P2A_mScarlet-I3
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE
Plasmid#20945Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsExpressionMammalianMutationH134R mutation in ChR2PromoterhSynAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-hChR2(H134R)-EYFP-WPRE
Plasmid#20942Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsExpressionMammalianMutationH134R mutation in ChR2PromoterEF1alphaAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-HOT_tdTomato
Plasmid#138567PurposeUniversal Cleavable Donor Plasmid for tagging with tdTomato using NHEJDepositorInserttdTomato
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSN067
Plasmid#101748PurposeSingle copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-FlicR1
Plasmid#74142PurposeMammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicatorDepositorInsertFlicR1
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC3.1_CMV_oROS-G
Plasmid#216111PurposeExpresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells.DepositorInsertoROS-G
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE
Plasmid#20943Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsmCherryExpressionMammalianMutationH134RPromoterCaMKIIaAvailable sinceJuly 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBABR IRFP-Bcd-LEXY
Plasmid#182595PurposeDrosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertBcd (bcd Fly)
UseTagsIRFP and LEXYExpressionInsectMutationPromotermaternal tubulinAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
FRB_nSuMMVp
Plasmid#118970PurposeExpresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domainDepositorInsertsplit nSuMMVp in fusion with FRB
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP_cSuMMVp
Plasmid#119182PurposeExpresses C-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FK-506 binding protein (FKBP)DepositorInsertsplit cSuMMVp in fusion with FKBP
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only