We narrowed to 1,214 results for: POLI

1. pFE-dabAB2 C539A

   Plasmid

   #133008

   Purpose

   pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a C539A mutation and a dabB2 gene  (Uniprot: D0KWS8)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C539A

   Promoter

   tetR

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFE-dabAB2 H524A

   Plasmid

   #133007

   Purpose

   pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a H524A mutation and a dabB2 gene (Uniprot: D0KWS8)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H524A

   Promoter

   tetR

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFE-dabAB2 D353A

   Plasmid

   #133006

   Purpose

   pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a D353A mutation and a dabB2 gene  (Uniprot: D0KWS8)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D353A

   Promoter

   tetR

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5A1-T

   Plasmid

   #92017

   Purpose

   Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV protease

   Depositor

   Brian Pfleger

   Article

   Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

   Inserts

   TALE targeting lac operator with 3 TEV cleavage sites
   Tobacco Etch Virus Protease
   

   Use

   TALEN

   Tags

   5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

   Expression

   Mutation

   S219V

   Promoter

   Available since

   Jan. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p5C3-T

   Plasmid

   #92022

   Purpose

   Expresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

   Depositor

   Brian Pfleger

   Article

   Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

   Inserts

   TALE targeting sucA with N-terminal TEV protease cleavage site
   Tobacco Etch Virus Protease
   

   Use

   TALEN

   Tags

   5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

   Expression

   Mutation

   S219V

   Promoter

   Available since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCellFree_G03 STAT1

   Plasmid

   #67116

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   STAT1 (STAT1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCellFree_G03 GTF2A1L

   Plasmid

   #67096

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GTF2A1L (GTF2A1L Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCellFree_G03 TP53

   Plasmid

   #67078

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TP53 (TP53 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCellFree_G03 IRF9

   Plasmid

   #67079

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   IRF9 (IRF9 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCellFree_G03 E-Cad

    Plasmid

    #67046

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    E-Cad (CDH1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCellFree_G03 TCEB2

    Plasmid

    #67049

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TCEB2 (ELOB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    M6R and Q115K

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCellFree_G03 BTF3L4

    Plasmid

    #67051

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BTF3L4 (BTF3L4 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

    Plasmid

    #71237

    Purpose

    Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A GFP
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. Watermelon backbone

    Plasmid

    #155258

    Purpose

    Can be used to generate a Watermelon library

    Depositor

    Aviv Regev, Joan Brugge

    Article

    Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSP1

    Plasmid

    #20060

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of deletion mutant (1-43) of human…

    Promoter

    Available since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-p58

    Plasmid

    #166942

    Purpose

    mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

    Insert

    p58 (Lman1 Rat)
    

    Use

    Tags

    GFP and chicken lysozyme signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRMC2-msfGFP

    Plasmid

    #194913

    Purpose

    Tetracycline inducible expression of msfGFP in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno Sequence followed by monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psicheck2 PTEN 3'UTR

    Plasmid

    #50936

    Purpose

    reporter for PTEN RNAi

    Depositor

    Pier Pandolfi

    Article

    Tay et al Cell. 2011 Oct 14;147(2):344-57. doi: 10

    Insert

    PTEN 3'UTR (PTEN Human)
    

    Use

    RNAi

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pX330 Human 3' HP1a gRNA

    Plasmid

    #127907

    Purpose

    WT Cas9 Vector targeting the 3' end of the human HP1a gene

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    gRNA for Human 3' HP1a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEf1a OsTIR ires NEO pA T2BH

    Plasmid

    #127910

    Purpose

    Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    OsTIR
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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