We narrowed to 15,941 results for: nans
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Plasmid#64067PurposeHuman cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).DepositorInsertDYNC1H1 (DYNC1H1 Human)
UseCre/LoxTagsHis-ZZ-TCS-SNAPfExpressionInsectMutationCodon optimized for Sf9Available SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(Y40C)
Plasmid#164903PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.DepositorInsertRaichuEV-Rac1 (RAC1 Human)
ExpressionMammalianMutationY40C mutation in the Rac1 domain whcih make the s…Available SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-NCR1-COMP5AP-AviTag-9xHis
Plasmid#157253PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Psicheck Cdk2ap1Exon1
Plasmid#178028Purposeexpression vector to test translation efficiency by luciferase reporter.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseLuciferase; NonviralTagsLuciferaseExpressionMammalianPromoterCloned inAvailable SinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15-ZmPYK
Plasmid#220230PurposeInducible expression of Zymomonas mobilis pyruvate kinaseDepositorTags6xHis-TEVExpressionBacterialPromoterT7Available SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hsChRmine-oScarlet-KV 2.1-WPRE
Plasmid#183521PurposeOptogeneticsDepositorInserthsChRmine-oScarlet-Kv2.1
UseAAVMutationH33RPromoterCaMKIIaAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFSF14-COMP5AP-AviTag-9xHis
Plasmid#157149PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-VSTM2A-Fc(DAPA)-AviTag-6xHis
Plasmid#156633PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertVSTM2A (VSTM2A Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3A-COMP5AP-AviTag-9xHis
Plasmid#157536PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFRSF12A-COMP5AP-AviTag-9xHis
Plasmid#157052PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-TRPS1-P2A-mCherry
Plasmid#203851PurposeTet-inducible lentiviral plasmid expressing TRPS1-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertTRPS1 (TRPS1 Human)
ExpressionMammalianAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-HLA-B-COMP5AP-AviTag-9xHis
Plasmid#157399PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SaPE2-P2A-BSD
Plasmid#174818PurposeMammalian expression of SaCas9 prime editor 2 with P2A-BSD markerDepositorInsertSaPE2-P2A-BSD
TagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVNP2.0-SpCas9
Plasmid#206880PurposeExpresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domainDepositorAvailable SinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-control
Plasmid#213788PurposeCIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-Calm3-U6-control gRNA
UseLentiviralTagsGFPExpressionMammalianPromoterSyn1, U6Available SinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3E-COMP5AP-AviTag-9xHis
Plasmid#157534PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVD1 Multiplexing Edit E3-E4-En-1 (GB2244)
Plasmid#160566PurposeGBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.DepositorInsertMultiplexing Edit (E3-E4-En-1)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-mCherry-Fc(DAPA)-AviTag-6xHis
Plasmid#156581PurposeMammalian expression of mCherry fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertmCherry
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-iGluSnFR4s
Plasmid#234443PurposeBacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow)DepositorInsertiGluSnFR4s
Tags6xHis-Xpress epiExpressionBacterialPromoterT7Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOD2046-bwmDEG
Plasmid#89367PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)PromoterPmyo-3Available SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only