We narrowed to 11,370 results for: VARS
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Plasmid#113617PurposeReporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFPDepositorInsertEJ7-GFP variant of EGFP
ExpressionMammalianAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-GFP-HiBit
Plasmid#170989PurposeFor bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAS-V5-TurboID-NES
Plasmid#116904PurposeExpresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.DepositorInsertTurboID
UseDrosophila expressionAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
P445-FBP_17
Plasmid#139708PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlledDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_1
Plasmid#78198PurposeExpression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A197C)
ExpressionBacterialMutationA197C (please see depositors comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-sbGLuc-B7-EYFP
Plasmid#114110Purposefusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogeneticsDepositorInsertsGaussia luciferase, slow burn
B7 hinge region
Enhanced Yellow Fluorescent Protein
UseAAVExpressionMammalianAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
ClCry4 Y319D
Plasmid#160649PurposeExpression of protein for various experiments including finding interaction partnersDepositorInsertClcry4 PHR Y319D
TagsHexa hisExpressionBacterialAvailable SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMALcPP-MS2cp
Plasmid#203351PurposeExpresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteriaDepositorInsertMS2 coat protein
TagsHx6-MBPExpressionBacterialMutationV75E and A81G, to prevent capsid assemblyAvailable SinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Phi3-GCaMP6f
Plasmid#106298PurposeCMV driven GFP based calcium (Ca2+) reporterDepositorInsertGCaMP6f
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_3
Plasmid#78200PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,I76G,A197C)
ExpressionBacterialMutationA17C,I76G,A197C (please see depositor comment bel…PromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME minprom KalTa4
Plasmid#195964PurposeGateway pME Gal4 variantDepositorInsertmouse beta-globin minimal promoter KalTA4 (Gal4)
UseOtherAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro SCRIB WT
Plasmid#88886PurposeSCRIB WT in MSCV backboneDepositorAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-GFAP-iGABASnFR2-WPRE
Plasmid#218871PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorHas ServiceAAV1 and AAV5InsertiGABASnFR2
UseAAVExpressionMammalianMutationS99A F102Y F104Y L178SPromoterGFAPAvailable SinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hRedOpsin-TGFB1
Plasmid#164427PurposeAAV plasmid expressing TGFB1 in photoreceptorsDepositorAvailable SinceNov. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AsCas12a-Plus-mCherry
Plasmid#191231PurposeTo express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificityDepositorInsertAsCas12a-Plus-P2A-mCherry
UseCRISPRMutationE174R, R951K, R955AAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
EP-Pol vector
Plasmid#131686PurposeA lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymeraseDepositorInsertAdenovirus DNA polymerase
UseLentiviralTagsHAMutationF421Y, D827AAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
ExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-iGABASnFR2-WPRE
Plasmid#218883PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseAAVExpressionMammalianMutationS99A F102Y F104Y L178SPromoterCAGAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluu
Plasmid#119834PurposeProduction of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluu
TagsGSTExpressionBacterialMutationS72TPromoterT7Available SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEL063
Plasmid#124895PurposeFor genome editing in plant by SpCas9 variant(SpCas9-NG)DepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only