We narrowed to 63,769 results for: cloning
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Plasmid#113983PurposeVector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectorsDepositorInsertGentamicin
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBP-Bba_K822000
Plasmid#74094PurposeL-Lactate inducible promoter from E. coli MG1655DepositorPublicationInsertBba_K822000 (L-Lactate promoter - E. coli MG1655)
UseSynthetic BiologyExpressionBacterialPromoterNAAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a-CA(A14C, E45C, A92E)
Plasmid#79046PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C, A92E)
ExpressionBacterialMutationA14C, E45C, A92E mutations in HIV-1 CA proteinPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-EGFP-NS-MCS
Plasmid#75180PurposeEGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-NS-MCS
UseThree prime entry vector for multisite gateway th…Promotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-SESN2
Plasmid#34820DepositorAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MTPN
Plasmid#34829DepositorAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MPP6
Plasmid#34806DepositorAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PEPD
Plasmid#31328DepositorAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EFS-MCS-WPRE
Plasmid#163362PurposeThe empty pLenti backbone with multiple cloning site that allows shuttling between pMY and pMX vectorsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNA500
Plasmid#149640Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFloat.cspA_v1
Plasmid#133071PurposeEntry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.DepositorTypeEmpty backboneUseGateway CloningTags3C-His6 and His6-3CExpressionBacterialPromotercspAAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
psgRNA2.02-PP7-ccdB
Plasmid#99912PurposeBasic cloning vector for the expression of PP7 inserted sgRNADepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/GCCACC-mEGFP-IRES-mCherry
Plasmid#49226PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOliSpec5'
Plasmid#228551PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmidDepositorInsertTruncated 5' region of aadA
UseCRISPRAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOliSpec3'
Plasmid#228550PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.DepositorInsertTruncated 3' region of aadA
UseCRISPRAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-NADAR
Plasmid#255737PurposeFor Gateway cloning of NADARDepositorAvailable sinceJuly 14, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDIV068
Plasmid#247961PurposeCloning vector containing A-PgpdA-PacI-Nt.BbvcI cassette-TtrpC-B sequences compatible with the DIVERSIFY gene landing platformDepositorTypeEmpty backboneUseSynthetic BiologyAvailable sinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
plix403-V5-N-mEYFP
Plasmid#223955PurposeGateway cloning of genes of interest with an N-terminal V5-mEYFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsV5-mEYFPAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAH46 - MosTI [unc-119(partial) | MCS | gfp]
Plasmid#200366PurposeCloning vector for mosTI(unc-119, gfp). MCS or Golden Gate.DepositorTypeEmpty backboneExpressionWormAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH45 - MosTI [unc-119(partial) | MCS | wrmScarlet]
Plasmid#200365PurposeCloning vector for mosTI(unc-119, wrmScarlet). MCS or Golden Gate.DepositorTypeEmpty backboneExpressionWormAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only