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RCH1:HDT1,HDT2-RNAi

Plasmid

#108446

Purpose

Arabidopsis transformation, RNAi vector targeting HDT1/HDT2 with RCH1 promoter

Depositor

Ton Bisseling

Article

Li et al Plant Cell. 2017 Sep;29(9):2183-2196. do

Inserts

HDT1 (HDA3 Mustard Weed)
HDT2 (HD2B Mustard Weed)

Use

RNAi

Expression

Plant

Promoter

RCH1

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only
ACS2

Plasmid

#13747

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

Acetyl CoA Sythetase 2 (Acss1 Mouse)

Tags

His

Expression

Bacterial

Mutation

Full-length ACS2

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
ACS2 Truncated

Plasmid

#13748

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

Truncated Acetyl CoA Sythetase 2 (Acss1 Mouse)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 39–682

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pNA0304

Plasmid

#165612

Purpose

Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)

Depositor

Marcus Noyes

Article

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)

Use

CRISPR

Expression

Yeast

Mutation

NotI site replaced the CAN1 spacer in parent vect…

Promoter

SNR52

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJK630

Plasmid

#72389

Purpose

Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate synthetase (AaPrsA-insV2)

Depositor

T. J. Kappock

Insert

phosphoribosylpyrophosphate synthetase

Expression

Bacterial

Mutation

inserted Val2

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pJK554

Plasmid

#72453

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJK631

Plasmid

#72390

Purpose

Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate synthetase (AaPrsAH6-insA2), C-terminal His6 tag

Depositor

T. J. Kappock

Insert

phosphoribosylpyrophosphate synthetase

Tags

His6

Expression

Bacterial

Mutation

inserted Ala2 to facilitate cloning

Promoter

T7

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJK531

Plasmid

#72512

Purpose

Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)

Depositor

T. J. Kappock

Insert

hypothetical protein in purSQL operon

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK241

Plasmid

#71693

Purpose

Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp404>Phe mutant (AarAH6-W404F)

Depositor

T. J. Kappock

Insert

citrate synthase

Tags

His6

Expression

Bacterial

Mutation

changed Tro404 to Phe

Promoter

T7 promoter

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pJK699

Plasmid

#71691

Purpose

Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Asp371>Asn mutant (AarAH6-D371N)

Depositor

T. J. Kappock

Insert

citrate synthase

Tags

His6

Expression

Bacterial

Mutation

changed Asp371 to Asn

Promoter

T7 promoter

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pJK708

Plasmid

#71692

Purpose

Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp400>Phe mutant (AarAH6-W400F)

Depositor

T. J. Kappock

Insert

citrate synthase

Tags

His6

Expression

Bacterial

Mutation

changed Trp400 to Phe

Promoter

T7 promoter

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pJK547

Plasmid

#72449

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide synthetase

Expression

Bacterial

Mutation

Val319 to Phe

Promoter

T7

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJK550

Plasmid

#72450

Purpose

Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins)

Depositor

T. J. Kappock

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

inserted Val2

Promoter

T7

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJK539

Plasmid

#72515

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, large subunit

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK549

Plasmid

#72520

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, large subunit

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK555

Plasmid

#72521

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK577

Plasmid

#72528

Purpose

Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, small subunit

Use

Gene synthesis

Promoter

n/a

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK532

Plasmid

#72513

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit with N-terminal His6 tag (H6AaPurQ)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, glutaminase subunit

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

March 17, 2016

Availability

Academic Institutions and Nonprofits only

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pJK544

Plasmid

#72517

Purpose

Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)

Depositor

T. J. Kappock

Insert

formylglycinamidine ribonucleotide synthetase, small subunit

Expression

Bacterial

Mutation

contains additional Ala2

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pJK546

Plasmid

#72518

Purpose

Produces Acetobacter aceti 1023 hypothetical protein in purSyQL cluster (AaOrfY)

Depositor

T. J. Kappock

Insert

hypothetical protein in purSQL operon

Expression

Bacterial

Mutation

contains additional Ala2

Promoter

T7

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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