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pAL004

Plasmid

#233863

Purpose

MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRNR2-rtTA-v16-tADH1; tetO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAL003

Plasmid

#233864

Purpose

MoClo-YTK URA multigene plasmid with MCS; Tet systems; pRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pRPL18B-rtTA3-tADH1; tetO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1_006_5C1SR

Plasmid

#235796

Purpose

5' Connector 1 Short Reverse in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 1 Short Reverse

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1_009_5C2SR

Plasmid

#235797

Purpose

5' Connector 2 Short Reverse in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 2 Short Reverse

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_1_019_5C7SR

Plasmid

#235800

Purpose

5' Connector 7 Short Reverse in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5' Connector 7 Short Reverse

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAPH07

Plasmid

#234345

Purpose

Library-scale IPTG-inducible gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK

Depositor

Jerome Fox

Article

Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12

Insert

Type II dCas9 sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

lacUV5

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAPH021

Plasmid

#234346

Purpose

Library-scale IPTG-inducible single transcript dual-gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK

Depositor

Jerome Fox

Article

Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12

Inserts

Type II dCas9 sgRNA
Type II dCas9 sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

lacUV5

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS∆P2

Plasmid

#225197

Purpose

Used to create a markerless deletion of the E. coli HS P2 phage

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

P2 phage homology arms

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7xTS_SpyCatcher:mNG

Plasmid

#225185

Purpose

Used for inserting SpyCatcher fused to mNeonGreen at the bacterial Tn7 site

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

SpyCatcher:mNeonGreen

Tags

SpyCatcher

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7xTS_SpyCatcher:AausFP1

Plasmid

#225186

Purpose

Used for inserting SpyCatcher fused to AausFP1 at the bacterial Tn7 site

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

SpyCatcher:AausFP1

Tags

SpyCatcher

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7xTS_SpyCatcher:mCitrine

Plasmid

#225187

Purpose

Used for inserting SpyCatcher fused to mCitrine at the bacterial Tn7 site

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

SpyCatcher:mCitrine

Tags

SpyCatcher

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7xTS_SpyCatcher:mTFP1

Plasmid

#225189

Purpose

Used for inserting SpyCatcher fused to mTFP1 at the bacterial Tn7 site

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

SpyCatcher:mTFP1

Tags

SpyCatcher

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7xTS_PrecA:mNeonGreen

Plasmid

#225192

Purpose

Used for inserting PrecA upstream of mNeonGreen at the bacterial Tn7 site for use as a RecA reporter

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

PrecA:mNeonGreen

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-FlpTag-DsRed-HDR-ph2

Plasmid

#231902

Purpose

dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 2.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

Frt-Frt14-SD-GFP-SA-Frt-Frt14

Expression

Bacterial

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-FlpTag-DsRed-HDR-ph1

Plasmid

#231901

Purpose

dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 1.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

Frt-Frt14-SD-GFP-SA-Frt-Frt14

Expression

Bacterial

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-FlpTag-DsRed-HDR-ph0

Plasmid

#231900

Purpose

dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 0.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

Frt-Frt14-SD-GFP-SA-Frt-Frt14

Expression

Bacterial

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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Dendra2-NS2B

Plasmid

#204218

Purpose

Expresses Dendra2-NS2B in Mammalian / Mouse Cell Lines

Depositor

Partha Mondal

Article

Aravinth et al Sci Rep. 2025 Mar 26;15(1):10358. doi: 1

Insert

Dengue (Dengue Type 2) NS2B (POLY Dengue Virus Serotype , Strain NGC)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WTSLFD

Plasmid

#232980

Purpose

Galactose iduced expression of Gcn4 WTSLFD+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WTSLFD+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

F108W, E109T, Y110S, E111L, N112F, L113D

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+HA

Plasmid

#232990

Purpose

Galactose iduced expression of Gcn4 Aro3+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro2+HA

Plasmid

#232989

Purpose

Galactose iduced expression of Gcn4 Aro2+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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