We narrowed to 28,875 results for: REP

1. AAVS1-SA-CAG-Pa01-BFP

   Plasmid

   #234142

   Purpose

   Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

   Depositor

   Gary Hon

   Article

   Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

   Insert

   Pa01
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCBE3-NG-HF1-Hypa

   Plasmid

   #218162

   Purpose

   This plasmid harbors the base editor SCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-NG-HF1-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / N497A…

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mGFP-hNET-A457P

   Plasmid

   #193365

   Purpose

   expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

   Depositor

   Harald Sitte

   Article

   Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

   Insert

   human Norepinephrine Transporter (A457P mutation) (SLC6A2 Human)
   

   Use

   Tags

   monomeric GFP (mGFP)

   Expression

   Mammalian

   Mutation

   A457P (GCC to CCC)

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mGFP-hNET-R121A/K334A/R440A

   Plasmid

   #193366

   Purpose

   expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

   Depositor

   Harald Sitte

   Article

   Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

   Insert

   human Norepinephrine Transporter (SLC6A2 Human)
   

   Use

   Tags

   monomeric GFP (mGFP)

   Expression

   Mammalian

   Mutation

   R121A (CGG to GCG) K334A (AAA to GCA) R440A (CGA …

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLED.RAB

   Plasmid

   #193015

   Purpose

   Photoreceptor-specific reporter (CBh promoter, Atp1b2 exon, bichromatic reporter)

   Depositor

   Jonathan Ling

   Article

   Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

   Insert

   Bichromatic reporter (EGFP and dsRed-Express2)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLD-puro-Cc-PARK7V51G-VA

   Plasmid

   #115183

   Purpose

   Lentiviral transduction and expression of PARK7V51G into any mammalian cell

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PARK7V51G (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   p.V51G

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLD-puro-Cc-PARK7C53A-VA

   Plasmid

   #115184

   Purpose

   Lentiviral transduction and expression of PARK7C53A into any mammalian cell

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PARK7C53A (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   p.C53A

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLD-puro-Cc-PARK7H126A-VA

   Plasmid

   #115185

   Purpose

   Lentiviral transduction and expression of PARK7H126A into any mammalian cell

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PARK7H126A (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   p.H126A

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLD-puro-Cc-PARK7E163K-VA

   Plasmid

   #115186

   Purpose

   Lentiviral transduction and expression of PARK7E163K into any mammalian cell

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PARK7E163K (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   p.E163K

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGK-Ldb3-mKate2

    Plasmid

    #128766

    Purpose

    Fluorescent mKate reporter for Ldb3

    Depositor

    Deepak Srivastava

    Article

    Stone et al Cell Stem Cell. 2019 Jul 3;25(1):87-102.

    Insert

    Ldb3 promoter (Ldb3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ldb3

    Available since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLY9

    Plasmid

    #130907

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-2G6, and Ptet

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLY59

    Plasmid

    #130927

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    sfgfp
    pspFΔHTH::MCP
    

    Use

    Synthetic Biology

    Tags

    ASV tag and MCP (MS2 coat protein)

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-2G6, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLY57

    Plasmid

    #130926

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA5B5, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLY56

    Plasmid

    #130925

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA4B4, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLY55

    Plasmid

    #130924

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA3B3, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLY53

    Plasmid

    #130911

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMiR-CIP2A-miR-375-mutC&D

    Plasmid

    #53712

    Purpose

    Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on miR-375 binding site C&D (refer t…

    Promoter

    Available since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pMiR-CIP2A-miR-375-mutE

    Plasmid

    #53710

    Purpose

    Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on miR-375 binding site E (refer to cita…

    Promoter

    Available since

    July 11, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pMiR-CIP2A-miR-375-mutA&B

    Plasmid

    #53711

    Purpose

    Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on miR-375 binding site A&B (refer t…

    Promoter

    Available since

    July 1, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pMiR-CIP2A-miR-375-mutD

    Plasmid

    #53709

    Purpose

    Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site

    Depositor

    Edward Chan

    Article

    Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation on miR-375 binding site D (refer to cita…

    Promoter

    Available since

    July 1, 2014

    Availability

    Academic Institutions and Nonprofits only