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pET23a_mod_RBPMS_FL

Plasmid

#59393

Purpose

Plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

6xHis, FLAG, and HA

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2015

Availability

Academic Institutions and Nonprofits only

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1201 pAdTrack FKHR H215R AAA

Plasmid

#9039

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR H215R AAA (FOXO1 Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

H215R T24A S256A S319A

Available Since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro U6 sgRNA SOX17 -91

Plasmid

#50923

Purpose

U6 driven sgRNA targeting Sox17 -91 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Sox17 -91 sgRNA (SOX17 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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NLS-dCas9-HA-NLS-GFP4-VPR

Plasmid

#183924

Purpose

VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP4-VPR

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMAL-c2X-ORF24-NTD

Plasmid

#138465

Purpose

Expresses MBP-tagged ORF24-NTD in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

MBP

Expression

Bacterial

Mutation

Amino acids 1-201

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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hisSUMO-Gli1

Plasmid

#87225

Purpose

Express hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coli

Depositor

Gary Stormo

Article

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

GLI1 (Gli1 Mouse)

Tags

hisSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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BirA-Myc_N_HME1

Plasmid

#167709

Purpose

Expresses HME1 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

HME1 (EN1 Human)

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-ORF24-NTD

Plasmid

#138464

Purpose

Expresses GST-tagged ORF24-NTD in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1-201

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFAB3725

Plasmid

#47837

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 6 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 6 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3869

Plasmid

#47842

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 11 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 11 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Eif4a1-R

Plasmid

#122345

Purpose

Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Eif4a1

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE HSF Flag Ac

Plasmid

#1949

Depositor

Robert Kingston

Article

Sullivan et al Mol Cell Biol 2001 Sep;21(17):5826-37.

Insert

Heat Shock Factor 1 (HSF1 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

Acidic mutations (D416, E493, E496 A)

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
-111 hTF m3

Plasmid

#15450

Depositor

Nigel Mackman

Article

Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

All 3 EGR-1 sites mutated

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-ORF24-3xFLAG

Plasmid

#138423

Purpose

Expresses full-length ORF24 with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Txnip

Plasmid

#178437

Purpose

doxycycline-inducible expression of Mouse Txnip in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

Txnip (Txnip Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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Pmyo-3::tag:SL1(G20A)

Plasmid

#81114

Purpose

Expression of exogenous SL1(G20A) in C. elegans muscle

Depositor

Xiao Liu

Article

Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

Insert

sls-1.2 (sls-1.2 Nematode)

Tags

illumina-adapter

Expression

Worm

Mutation

G20A

Promoter

promoter of myo-3

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.mTagBFP2.let-7c

Plasmid

#169310

Purpose

Constitutive overexpression of let-7c with mTagBFP2 as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

let-7c (MIRLET7C Human)

Use

Lentiviral

Tags

TagBFP2

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFAB3992

Plasmid

#47823

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD16 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD16

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a His6Flag2AUF1(p42)

Plasmid

#135941

Purpose

Expresses AUF1p42 in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Tags

Flag2 and His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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LacI/109-pHG165c

Plasmid

#90058

Purpose

WT LacI polymorphism with T at position 109

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LacI/109

Expression

Bacterial

Mutation

109

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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