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We narrowed to 9,223 results for: Coli

Showing: 1001 - 1020 of 9223 results

  1. pJK500

    Plasmid
    #72245
    Purpose
    Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…
    Promoter
    T7 and endogenous
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72245-plasmid-map-sequence-id-140434

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  2. pHL 1571

    Plasmid
    #37760
    Depositor
    Han Lim
    Article
    Hussein et al Nucleic Acids Res. 2012 May 22.
    Inserts
    ptet_ompC_gfpAaV
    pLac_st3 TetR_mCherry
    Use
    Synthetic Biology; E. coli
    Tags
    gfp and mCherry
    Expression
    Mutation
    AaV degradation tag added to the 3'end
    Promoter
    pLac and ptet
    Available since
    Aug. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  3. pHL 1569

    Plasmid
    #37759
    Depositor
    Han Lim
    Article
    Hussein et al Nucleic Acids Res. 2012 May 22.
    Inserts
    ptet_ompC_gfpAaV
    pLac_micC_mCherry
    Use
    Synthetic Biology; E. coli
    Tags
    gfp and mCherry
    Expression
    Mutation
    AaV degradation tag added to the 3'end
    Promoter
    pLac and ptet
    Available since
    July 27, 2012
    Availability
    Academic Institutions and Nonprofits only

  4. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

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  6. pRelA*

    Plasmid
    #175590
    Purpose
    Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Use
    Tags
    Fused with a flexible GS linker to mVenus
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175590-plasmid-map-sequence-id-348362

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  8. pMis1_1B

    Plasmid
    #192079
    Purpose
    A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction
    Depositor
    Markus Buchhaupt
    Article
    Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.
    Inserts
    KanR
    rhaR
    rhaS
    pBBR1 rep
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192079-plasmid-map-sequence-id-380008

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  10. pETM6-FAS

    Plasmid
    #49810
    Purpose
    Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configuration
    Depositor
    Mattheos Koffas
    Article
    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc
    Inserts
    fatty acyl-ACP thioesterase
    hydroxydecanoyl-ACP dehydratase
    b-ketoacyl-ACP synthase III
    3-ketoacyl-ACP reductase
    enoyl-(acyl carrier protein) reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon-optmized. See supplemental information of …
    Promoter
    T7
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49810-plasmid-map-sequence-id-74625

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  12. pYihI-N+C-term K-R

    Plasmid
    #233093
    Purpose
    Expression of GST-YihI with N+C-term lysines (K) mutated to arginine (R)
    Depositor
    Michael Downey
    Article
    Baijal et al bioRxiv 2025.02.12.637445
    Insert
    GST-YihI with N+C-term lysines (K) mutated to arginine (R)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    GST-YihI with N+C-term lysines (K) mutated to arg…
    Promoter
    Available since
    May 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233093-plasmid-map-sequence-id-472239

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  13. pRAR101

    Plasmid
    #185046
    Purpose
    J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region with amino acids 2-8 deleted, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31p
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Amino acids 2-8 deleted of L31p coding region
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185046-plasmid-map-sequence-id-363352

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  14. pRAR111

    Plasmid
    #185047
    Purpose
    J23108(spel), pHP14 stability hairpin, strong RBS, L31p coding region with last 8 amino acids deleted, trrnB
    Depositor
    Julius Lucks
    Article
    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127
    Insert
    L31p
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Last 8 amino acids deleted of L31p coding region
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    185047-plasmid-map-sequence-id-363355

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  16. pSMART-Ala2

    Plasmid
    #71326
    Purpose
    NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Article
    Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.
    Insert
    NADPH Alanine Dehydrogenase (ald Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D196A, L197R
    Promoter
    insulated waaHp from E. coli
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    71326-plasmid-map-sequence-id-132780

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  17. pVR2

    Plasmid
    #84719
    Purpose
    E. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcription
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    12 nt stretch lacking A's inserted before 5&…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84719-plasmid-map-sequence-id-167481

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  18. His8:MBP-tev-Ala-eK-Flv

    Plasmid
    #83393
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Article
    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83393-plasmid-map-sequence-id-174595

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  19. His8:MBP-tev-Ile-eK-Flv

    Plasmid
    #83392
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Article
    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83392-plasmid-map-sequence-id-174594

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  20. His8:MBP-tev-Tyr-eK-Flv

    Plasmid
    #83391
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Article
    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83391-plasmid-map-sequence-id-174593

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Showing: 1001 - 1020 of 9223 results