We narrowed to 24,459 results for: Spr

1. pLenti-multi-CRISPR-sgMlkl_#1-puro

   Plasmid

   #231979

   Purpose

   Knockout mouse Mlkl

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 with puromycin resistance (Mlkl Mouse)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiCRISPRv2puro-E-cadherin(Canis)-exon1 6-28 gRNA

   Plasmid

   #209922

   Purpose

   A knock-out vector for the dog CDH1

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   A gRNA targeting the dog CDH1 gene.
   

   Use

   CRISPR and Lentiviral

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-HsProm1s1(W795R)-TwinStrep-His

   Plasmid

   #210817

   Purpose

   Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (W795R)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Tags

   TwinStrep, 10xHis

   Expression

   Mammalian

   Mutation

   Changed Trp-795 to Arg

   Promoter

   CMV

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Anti-CASPR/Neurexin IV [K65A/30R]

   Plasmid

   #219448

   Purpose

   Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat) IgG2a R-mAb. Derived from hybridoma K65A/30.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant mouse monoclonal antibody. (Cntnap1 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Anti-CASPR/Neurexin IV [K65A/43R]

   Plasmid

   #219449

   Purpose

   Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat) IgG2a R-mAb. Derived from hybridoma K65A/43.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant mouse monoclonal antibody. (Cntnap1 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Anti-CASPR/Neurexin IV [K65/35R-1]

   Plasmid

   #206683

   Purpose

   Mammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat). Derived from hybridoma K65/35-1.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-CASPR/Neurexin IV (Rattus norvegicus) recombinant Mouse monoclonal antibody (Cntnap1 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Anti-CASPR/Neurexin IV [K65/35R-2b]

   Plasmid

   #199403

   Purpose

   Mammalian expression plasmid of Anti-CASPR/Neurexin IV [K65/35R-2b] (Rat). Derived from hybridoma K65/35.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-CASPR/Neurexin IV (Rat) recombinant mouse monoclonal antibody (Cntnap1 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPRv2-sgRNAs-del-ZNF91-BFP

   Plasmid

   #202823

   Purpose

   Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and BFP expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   two sgRNAs (each with a U6 promoter) to delete exon 1 within ZNF91 gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 - two

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA17-CRISPR-Tag (11XTS1) for dSpCas9

   Plasmid

   #199448

   Purpose

   CRISPR-Tag sequence that can be efficiently bound by dSpCas9

   Depositor

   Baohui Chen

   Article

   Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

   Insert

   CRISPR-Tag
   

   Use

   CRISPR

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. RPS4X sgRNA-3 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192233

    Purpose

    lentiviral vector expressing sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. RPS15A sgRNA-5 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192230

    Purpose

    lentiviral vector expressing sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-5 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. RPS4X sgRNA-4 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192234

    Purpose

    lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. RPS15A sgRNA-4 lentiCRISPR v2-Blast plasmid

    Plasmid

    #192229

    Purpose

    lentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-4 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

    Plasmid

    #176836

    Purpose

    Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
    

    Use

    Unspecified

    Mutation

    See Depositor Comments Below

    Promoter

    Tet operator (tetracycline inducible expression)

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid

    #185912

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid

    #185917

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid

    #197511

    Purpose

    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    

    Use

    AAV and CRISPR

    Tags

    BPNLS and BPNLS-NpuC(intein)

    Mutation

    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…

    Promoter

    Cbh

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid

    #139997

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

    Plasmid

    #223125

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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