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We narrowed to 23,774 results for: Spr

Showing: 1001 - 1020 of 23774 results

  1. pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

    Plasmid
    #221447
    Purpose
    Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus
    Depositor
    Daniel Durocher
    Article
    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.
    Insert
    NFATC2IP (NFATC2IP Human)
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    SPR degron: hGEM (1-110) fragment that is fused t…
    Promoter
    CMV
    Available since
    July 3, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221447-plasmid-map-sequence-id-437531

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  8. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

    Plasmid
    #62580
    Purpose
    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.
    Depositor
    Michael Ruppert
    Article
    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6
    Insert
    SPRED1 (SPRED1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    miR-21 binding site 1 mutant (TAAGCTA --> TAGA…
    Promoter
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

    Plasmid
    #62581
    Purpose
    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.
    Depositor
    Michael Ruppert
    Article
    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6
    Insert
    SPRED1 (SPRED1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    miR-21 binding site 2 mutant (TAAGCTA --> TAGA…
    Promoter
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid
    #85756
    Purpose
    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Inserts
    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    Use
    CRISPR
    Tags
    Csy4, Csy4 recognition sequence, FokI fusion via …
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CMV and U6
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85756-plasmid-map-sequence-id-170275

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  12. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

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  13. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid
    #138148
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NES
    Expression
    Mutation
    Promoter
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138148-plasmid-map-sequence-id-318593

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  14. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid
    #138143
    Purpose
    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PguCas13b
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138143-plasmid-map-sequence-id-275400

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  17. pCMV-T7-BPNLS-CE-ABE8e-SpRY--BPNLS-P2A-EGFP (NK289)

    Plasmid
    #208293
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpRY A-to-G base editor and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-CE-ABE8e-SpRY-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA inlaid into nSpRY(D10A/A6…
    Promoter
    CMV and T7
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208293-plasmid-map-sequence-id-410845

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  18. pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

    Plasmid
    #138145
    Purpose
    Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PspCas13b
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138145-plasmid-map-sequence-id-272531

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  19. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

    Plasmid
    #138146
    Purpose
    Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PspCas13b
    Use
    Lentiviral
    Tags
    NES
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138146-plasmid-map-sequence-id-272533

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  20. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

    Plasmid
    #138144
    Purpose
    Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PguCas13b
    Use
    Lentiviral
    Tags
    NES
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138144-plasmid-map-sequence-id-272529

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Showing: 1001 - 1020 of 23774 results