We narrowed to 165,256 results for: addgene

1. AAV pCAG-FLEX-mScarlet-WPRE

   Plasmid

   #99280

   Purpose

   Can be used to generate AAV virus that will express mScarlet in the presence of Cre

   Depositor

   Rylan Larsen

   Insert

   mScarlet
   

   Use

   AAV and Cre/Lox

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOK12

   Plasmid

   #49789

   Purpose

   E. coli cloning vector (KanR, low copy, blue/white selection)

   Depositor

   Joachim Messing

   Article

   Vieira et al Gene. 1991 Apr;100:189-94.

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   lacZ

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. 7TFP

   Plasmid

   #24308

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-FFluc // SV40-PuroR
   

   Use

   Lentiviral

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABEmax-SpRY

   Plasmid

   #195279

   Purpose

   Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP

   Depositor

   Carsten Lederer, Petros Patsali

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-FLEXfrt-SaCas9-U6-sgRosa26

   Plasmid

   #159915

   Purpose

   Mutagenesis of Rosa26

   Depositor

   Larry Zweifel

   Insert

   Rosa26 (Gt(ROSA)26Sor Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLIK CXADR-V5 hygro

   Plasmid

   #220362

   Purpose

   Lentiviral expression vector for an inducible V5-tagged CXADR construct

   Depositor

   Kevin Janes

   Insert

   CXADR (CXADR Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV CMV IGF1-FLAG

   Plasmid

   #175170

   Purpose

   Lentiviral expression of human Igf1-FLAG

   Depositor

   Larry Gerace

   Insert

   Igf1 (IGF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9_human_ADAR1_ccC

   Plasmid

   #158115

   Purpose

   deletion hADAR1

   Depositor

   Emanuela Felley-Bosco

   Insert

   Adar1 (ADAR Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9_human_ADAR1_ccB

   Plasmid

   #158116

   Purpose

   deletion hADAR1

   Depositor

   Emanuela Felley-Bosco

   Insert

   Adar1 (ADAR Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mu-FLT3Lv1-pTRIP

    Plasmid

    #163519

    Purpose

    Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells

    Depositor

    Thomas Weber

    Insert

    murine-FLT3L variant 1 (Flt3l Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-CRISPR-Puro

    Plasmid

    #208646

    Purpose

    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

    Expression

    Mammalian

    Promoter

    CMV and U6 in tandem

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNR4A1.1.0-gDNA

    Plasmid

    #112391

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1

    Depositor

    Kevin White

    Insert

    NR4A1 (NR4A1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PERK 7: PERK.K618A.9E10.pBABE

    Plasmid

    #21820

    Depositor

    David Ron

    Insert

    PERK K618A (Eif2ak3 Mouse)
    

    Use

    Retroviral

    Tags

    Myc (9E10)

    Expression

    Mammalian

    Mutation

    kinase dead K618A mutant

    Available Since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. p226-AAV-dlx-dio-mScarlet-T2A-SYPEGFP

    Plasmid

    #185696

    Purpose

    AAV vector expressing cre-dependent mem-mScarlet, T2A, Synaptophysin-EGFP driven by Dlx promoter

    Depositor

    Lynette Lim

    Insert

    mScarlet, T2A, Synaptophysin-EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    mScarlet palmitoylation, Synaptophysin fused to E…

    Expression

    Mammalian

    Promoter

    mDlx

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SETD1A

    Plasmid

    #32868

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SETD1A (SETD1A Human)
    

    Tags

    His tag (with TEV cleavage)

    Expression

    Bacterial

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

16. SND1 (SND1A-c003)

    Plasmid

    #98242

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SND1A (SND1 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00567:TUDOR 677-799

    Promoter

    T7

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. 6VAH

    Plasmid

    #162273

    Purpose

    Bacterial Expression

    Depositor

    Cheryl Arrowsmith

    Insert

    TEAD2 (TEAD2 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. XLone-Puro NFIL3 P2A eGFP NLS

    Plasmid

    #140026

    Purpose

    Tunable and temporal expression control of NFIL3 and nuclear eGFP

    Depositor

    Xiaoping Bao

    Insert

    NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-FLAG-mMdm2-IRES-GFP

    Plasmid

    #66955

    Purpose

    Retroviral vector to express a FLAG-tagged mouse Mdm2

    Depositor

    Martine Roussel

    Insert

    Mdm2 (Mdm2 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

20. integrin alpha9 pBabe puro

    Plasmid

    #13604

    Depositor

    Dean Sheppard

    Insert

    integrin alpha 9 (ITGA9 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    contains mature ITGA9 (aa30-1035 compared to NP_0…

    Available Since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only