We narrowed to 4,849 results for: lenti sgrna

1. psPAX2-D64V-NC-COM

   Plasmid

   #131226

   Purpose

   Expressing GAG precursor protein, in which COM is inserted after the second zinc finger domain of nucleocapsid protein (NC)

   Depositor

   Baisong Lu

   Article

   Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

   Insert

   COM
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM808

   Plasmid

   #134181

   Purpose

   Lentiviral sgRNA plasmid with blasticidin resistance

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSP618

   Plasmid

   #169235

   Purpose

   Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

   Depositor

   William Greenleaf

   Article

   Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

   Insert

   mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO-CreERT2 stuffer v3

   Plasmid

   #158046

   Purpose

   lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLKO-Cre stuffer v4

   Plasmid

   #158032

   Purpose

   lenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLKO-Cre sgAmotl2 v3

   Plasmid

   #158602

   Purpose

   lenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   Amotl2 sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLKO-Cre sgAdam10 v3

   Plasmid

   #158034

   Purpose

   lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   Adam10 sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pLKO-Cre sgUroD v4

   Plasmid

   #158036

   Purpose

   lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse UroD

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   UroD sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLKO-Cre sgItgb5 v3

   Plasmid

   #158038

   Purpose

   lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Itgb5

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   Itgb5 sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLKO-Cre sgRipk4 v3

    Plasmid

    #158035

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Ripk4

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Ripk4 sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. JPF0464

    Plasmid

    #124035

    Purpose

    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a human non-targeting sgRNA in a 2nd gen. Lenti Virus destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pHR_pEF1a-iRFP713_non targeting sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. JPF0465

    Plasmid

    #124036

    Purpose

    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a mScarlet-targeting sgRNA in a 2nd gen. Lenti Virus destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pHR_pEF1a-iRFP713_mScarlet sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. JPF0466

    Plasmid

    #124037

    Purpose

    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a tagBFP-targeting sgRNA in a 2nd gen. Lenti Virus destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pHR_pEF1a-iRFP713_tagBFP sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. JPF0468

    Plasmid

    #124038

    Purpose

    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of tagBFP- and mScarlet-targeting sgRNAs in a 2nd gen. Lenti Virus destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pHR_pEF1a-iRFP713_mScarletsgRNA_tagBFP sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-CRISPR-Puro

    Plasmid

    #208646

    Purpose

    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX_311-Cas9

    Plasmid

    #96924

    Purpose

    for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

    Depositor

    David Root, John Doench, William Hahn

    Article

    Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    3xFlag and V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDECKO_mCherry

    Plasmid

    #78534

    Purpose

    Backbone to clone single or paired sgRNAs

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA)

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZR060_CROPseq-sgCtrl-CRISPRi

    Plasmid

    #180269

    Purpose

    Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    No-Target Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZR069_CROPseq-sgPTPRC-CRISPRi

    Plasmid

    #180271

    Purpose

    Lentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRi

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    PTPRC (CD45) sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pspCas9-3'UTR-Tetra-com-vector

    Plasmid

    #132553

    Purpose

    Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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