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Showing: 10181 - 10200 of 16420 results

  1. pGEX-4T-3-cytXlATL2

    Plasmid
    #124063
    Purpose
    Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.
    Depositor
    Tom Rapoport
    Article
    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d
    Insert
    cytXIATL2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    124063-plasmid-map-sequence-id-243791

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  3. pACEBAC1-CoREST-C

    Plasmid
    #109159
    Purpose
    Encodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Monika Golas
    Article
    Inui et al Protein Sci. 2017 May;26(5):997-1011. do
    Insert
    human C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
    Use
    Tags
    3xFLAG
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    Jan. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    109159-plasmid-map-sequence-id-232887

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  7. pcDNA5-hygro-eGFP-STOP-HEC1

    Plasmid
    #114026
    Purpose
    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.
    Depositor
    Geert Kops
    Article
    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1
    Insert
    eGFP-STOP-HEC1 (NDC80 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Start codon of Hec1 is mutated to stop codon
    Promoter
    Available since
    Sept. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    114026-plasmid-map-sequence-id-221030

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  13. pAM5081

    Plasmid
    #85107
    Purpose
    Ptrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.
    Depositor
    Susan Golden
    Article
    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d
    Insert
    YFP-KaiC
    Use
    Tags
    YFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85107-plasmid-map-sequence-id-180104

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  14. GST-RP2-C86Y,P95L

    Plasmid
    #86074
    Purpose
    A bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    RP2 (RP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Changed cysteine-86 and proline-95 to tyrosine an…
    Promoter
    tac
    Available since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    86074-plasmid-map-sequence-id-177989

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  16. pPD3cC

    Plasmid
    #86972
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …
    Promoter
    T7 promoter
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86972-plasmid-map-sequence-id-173929

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Showing: 10181 - 10200 of 16420 results