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pGEX6p-1-CYB5R3 deltaN26

Plasmid

#204557

Purpose

Expresses GST-tagged CYB5R3 deltaN26 in E.coli

Depositor

Masaaki Komatsu

Publication

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Expression

Bacterial

Mutation

delta1-26

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.0-Pct5.1-crRNA(AarI)

Plasmid

#191633

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloning

Depositor

Emily Balskus

Publication

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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DDX24

Plasmid

#155512

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX24 (DDX24 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV5 KSR1 T274V

Plasmid

#25975

Depositor

Rob Lewis

Publication

Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed threonine 274 to valine

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
pCMV5 MYC-Smurf2 (FF29/30A, Y459A) WT

Plasmid

#24606

Depositor

Jeff Wrana

Publication

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A, Y459A

Available since

May 5, 2010

Availability

Industry, Academic Institutions, and Nonprofits
3xDHFR-myc

Plasmid

#20216

Depositor

Carolyn Bertozzi

Publication

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xDHFR (folA E. coli)

Tags

myc

Expression

Mammalian

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-HYlight(low)

Plasmid

#255886

Purpose

Expresses HYlight(low) cytosolically in mammalian cells

Depositor

Alison Tebo

Publication

Tyler et al bioRxiv 2026.04.29.721630

Insert

HYlight2-low

Expression

Mammalian

Mutation

G92D, G125V, V143M, S267C, S398T, T416I, K498R, S…

Promoter

CMV

Available since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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pET28a-HaloTag-CNA35

Plasmid

#215457

Purpose

Encodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.

Depositor

Johanna Ivaska

Publication

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

CNA35

Tags

HaloTag

Expression

Bacterial

Mutation

Includes stop codon

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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myr-palm-mCherry2

Plasmid

#197910

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Publication

Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

myristoylated-palmitoylated-mCherry2

Expression

Mammalian

Mutation

none

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ6-LacI-Ptac(LacO)-LacZ

Plasmid

#191621

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X

Depositor

Emily Balskus

Publication

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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ADD1

Plasmid

#155397

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ADD1 (ADD1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CSTF1

Plasmid

#155485

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CSTF1 (CSTF1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 SARS-CoV-2 S-frag5_nostop

Plasmid

#153177

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag5

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pRS426-TUB1-internal6xHis

Plasmid

#60390

Purpose

Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter

Depositor

Ron Vale

Publication

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4)

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
NHA- Delta 5

Plasmid

#36440

Depositor

Edward Chan

Publication

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TNRC6A Delta 5 (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

aa 1670-1962 only

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pQE60 R130A wgMDH

Plasmid

#204266

Purpose

Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Publication

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R130A

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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DMPK

Plasmid

#155550

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DMPK (DMPK Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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GLE1

Plasmid

#155625

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

GLE1 (GLE1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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FUBP1

Plasmid

#155613

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

FUBP1 (FUBP1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CPSF4-1

Plasmid

#155478

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPSF4 (CPSF4 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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