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PKC beta II dNPS

Plasmid

#16383

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: aa 30…

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
FXR1

Plasmid

#25341

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

FXR1_25 (FXR1 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
Ac5-STABLE1-puro

Plasmid

#208031

Purpose

Enables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSBDEST.H

Plasmid

#79464

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Hygromycin selection

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-ZKSCAN1 nt 400-1782-mMALAT1_3' Sense

Plasmid

#60647

Purpose

Expresses the human ZKSCAN1 exon 2/3 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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mCerulean3-PMP-N-10

Plasmid

#55439

Purpose

Localization: Peroxisomes, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

PMP (PXMP2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean3-Paxillin-22

Plasmid

#55436

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Paxillin

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pV1329

Plasmid

#111438

Purpose

Solo vector pV1326 +sgCgADE2

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgCgADE2

Use

CRISPR

Expression

Yeast

Available Since

Dec. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGGF002

Plasmid

#48843

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::BastaR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site in 35S promoter re…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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mVermilion-C1

Plasmid

#105756

Purpose

Empty vector for creating N terminal mVermilion fusions

Depositor

Megan Rizzo

Article

Lee et al Elife. 2021 Oct 29;10. pii: 67858. doi:

Type

Empty backbone

Tags

mVermilion

Expression

Mammalian

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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Luc-Pten-3'UTR

Plasmid

#64098

Purpose

Pten Luciferase reporter

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

Pten 3'UTR (PTEN Human)

Expression

Mammalian

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only
Thy1.2-NLS_HA_Dre-P2A-BFP

Plasmid

#51275

Purpose

Co-expression of the site-specific recombinase Dre and BFP under the control of the Thy1.2 expression cassette

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS_HA_Dre-P2A-BFP

Promoter

Thy1.2

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pISH-Satb1

Plasmid

#74357

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Satb1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

special AT-rich sequence binding protein 1 (Satb1 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pGL-MLKif3B

Plasmid

#13744

Depositor

Linda Wordeman

Article

Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

Insert

Motorless Kif3B (Kif3b Mouse)

Use

Cre/Lox

Tags

GFP

Expression

Mammalian

Available Since

Jan. 29, 2007

Availability

Academic Institutions and Nonprofits only

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pBT316_pattB-pCA-GFP-pA

Plasmid

#52548

Purpose

for Phic31 integrase-mediated transgene integration

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCMB-AUTr

Plasmid

#61205

Purpose

Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LC3(ATG8)

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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NbALFA-TEV219

Plasmid

#201487

Purpose

TANGO detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbALFA-TEV219

Tags

TEV219

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 Y128F

Plasmid

#61734

Purpose

Expresses human Y128F NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

None

Expression

Mammalian

Mutation

Y128F

Promoter

Ef1a

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pH3FS-PYK1

Plasmid

#85781

Purpose

N-ICE plasmid pH3FS-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL70002

Plasmid

#50333

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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