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pPSP6

Plasmid

#48150

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6-miR-30 L1-R5

Plasmid

#62113

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;tmem88aCE1-P1Egfp

Plasmid

#90148

Purpose

contains tmem88a enhancer sequence, and tmem88a specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

tmem88aE1P1Egfp (tmem88a Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISP_IS

Plasmid

#120425

Purpose

This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

Depositor

Tamás Fehér

Article

Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

Insert

CRISPR spacers targeting IS1, IS5, IS3

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

constitutive

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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REEP5-2Strep

Plasmid

#210350

Purpose

Expresses REEP5 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP5 (REEP5 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR sgSHMT1_1

Plasmid

#106311

Purpose

Express Cas9 and sgRNA targeting SHMT1

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting SHMT1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-UVRAG CC

Plasmid

#24400

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

UVRAG (UVRAG Human)

Tags

His

Expression

Bacterial

Mutation

Contains on the CC domain, amino acids 200-270

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
p4118

Plasmid

#198151

Purpose

bacterial expression of sseF::HaloTag fusion

Depositor

Michael Hensel

Article

Goser et al Nat Commun. 2023 Mar 4;14(1):1240. doi:

Insert

PsseA::sscB sseF::L16::HaloTag::HA (sseF Salmonella enterica serovar Typhimurium)

Tags

HaloTag-HA

Expression

Bacterial

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter TCF (1) site mutant pGL3Basic

Plasmid

#32733

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase

Mutation

-75 TCF(1) site CTTTGAT deletion

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
L_NES 67kDa

Plasmid

#201357

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Adeno_NES-EGFP-6PrA

Expression

Mammalian

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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M_NLS 67kDa

Plasmid

#201351

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-6PrA

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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H_NES 67kDa

Plasmid

#201350

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

HIV_NES-EGFP-6PrA

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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M_NES 67kDa

Plasmid

#201356

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

MAPK_NES-EGFP-6PrA

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-syn-fRCaMP1

Plasmid

#125245

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMP1

Expression

Mammalian

Mutation

W44Y

Promoter

synapsin

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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p47phox-PX (1-122)

Plasmid

#119124

Purpose

Bacterial expression of human phox homology (PX) domain, p47phox-PX (1-122)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

p47phox-PX (1-122)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP

Plasmid

#199361

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOstar-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP

Plasmid

#199362

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-SUMOstar-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSilencer2.1-U6-LRS1F50C

Plasmid

#190856

Purpose

For mammalian expression of LRS1F50C, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer is regulated by riboswitch elements.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

LRS1F50C (A Dual-Specificity aptamer targeting OGT and GFP)

Use

Affinity Reagent/ Antibody and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSLiP-G1

Plasmid

#188963

Purpose

L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

dCas9
sgRNA: atcggtcgcattgttttccactagg

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSYN-VWF-039

Plasmid

#120292

Purpose

VWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion protein

Depositor

Robert Peters

Article

Dong et al Blood. 2019 Jan 14. pii: blood-2018-10-8

Insert

VWF D1-D2-D'-D3-Fc

Tags

Fc

Expression

Mammalian

Mutation

C1099A/C1142A

Promoter

CMV

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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