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Plasmid#74940PurposeUbiquitin variant with mutations L69P and V70L reverted to wild type (i53-DM mutant)DepositorInsertUbiquitin
UseTagsFlagExpressionMammalianMutationUbvG08 with I44A, P69L, and L70V mutations and no…PromoterCMVAvailable sinceMay 13, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL.EZH2
Plasmid#125171PurposeExpresses human EZH2 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorInsertEZH2 (EZH2 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-R536C-WLS-HA-IRES-GFP
Plasmid#178065PurposeExpression of WLS-HA protein (R536C mutant)DepositorInsertWLS (WLS Human)
UseTagsHA and IRES-GFPExpressionMammalianMutationR536CPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pvalb-2A-Dre Flp-in replacement vector
Plasmid#61571PurposeRecombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codonDepositorInsertPvalb-2A-Dre (Pvalb D6 Bacteriophage, Mouse)
UseRecombinase-mediated cassette exchange using flp …TagsExpressionMutationPromoterAvailable sinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_WW-PolyA
Plasmid#112290PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDADepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with mutations in the WW domain…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1-2
Plasmid#52378Purposeexpresses human Syndecan-1 GAGAL replaced with GADEV of SDC2 in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationGAGAL of syndecan-1 replaced with of GADED of SDC…PromoterCMVAvailable sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1-4
Plasmid#52375Purposeexpresses human Syndecan-1 GAGAL replaced with GDLDD of SDC4 in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationGAGAL of syndecan-1 replaced with GDLDD of SDC4; …PromoterCMVAvailable sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTK41
Plasmid#236110PurposeExpresses the motor domain of rat KIF5C fused with SNAP tag and ALFA tag nanobodyDepositorInsertsUseTagsALFA tag nanobody, His tag, SNAP tag, and StrepII…ExpressionBacterialMutationC7S mutation is introduced. 1-430 aa is encoded.PromoterT7Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBIG1e-CDK12deltaNTD /Cyclin K
Plasmid#231741PurposeProtein expression in insect cellsDepositorUseTagsFlag-3CExpressionInsectMutationCDK12(700-1490)PromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBIG1e-CDK12deltaCTD/Cyclin K
Plasmid#231742PurposeProtein expression in insect cellsDepositorUseTagsFlag-3CExpressionInsectMutationCDK12(1-1082)PromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only