We narrowed to 11,595 results for: Emb;
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Plasmid#215934PurposeFragmid fragment: (guide cassette) reverse orientation; guide expression; positive control cell surfaceDepositorHas ServiceCloning Grade DNAInsertrev{U6_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs]}
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MRGPRX3-DuET
Plasmid#213346PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MRGPRE-DuET
Plasmid#213341PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-SRRD-V5-APEX2 (in pLEX_307)
Plasmid#214921Purposeconstitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylationDepositorAvailable SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-AnkG ZU5in-3xHA
Plasmid#213730PurposeMammalian cell expression vector for overexpression of AnkG ZU5in isoform in motor neurons.DepositorInsertAnkG ZU5in (Ank3 Mouse)
ExpressionMammalianAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIDAF
Plasmid#213710PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTST-EGFP-GPIBY55
Plasmid#213706PurposeExpress the affinity sorting tag TST-EGFP- GPIBY55.DepositorInsertEGFP
TagsTwin-strep-tagExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTST-EGFP-GPICEAM7
Plasmid#213705PurposeExpress the affinity sorting tag TST-EGFP- GPICEAM7.DepositorInsertEGFP
TagsTwin-strep-tagExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTST-EGFP-GPIDAF
Plasmid#213704PurposeExpress the affinity sorting tag TST-EGFP- GPIDAF.DepositorInsertEGFP
TagsTwin-strep-tagExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-AnkG ZU5ex-3xHA
Plasmid#213731PurposeMammalian cell expression vector for overexpression of AnkG ZU5ex isoform in motor neurons.DepositorInsertAnkG ZU5ex (Ank3 Mouse)
ExpressionMammalianAvailable SinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-Topo-kcnk19/kcnk12l
Plasmid#209001PurposeRiboprobe generationDepositorAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(ERED198-201AAAA)-3xFLAG-pCDNA
Plasmid#201965PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorAvailable SinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMX1463
Plasmid#208805PurposeExpresses plasma-membrane-bound FRB-SBFP2 for rapid relocalization of cytoplasmic proteins tagged with FKBP upon addition of rapamycinDepositorAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETmsGFPSyt1(80 JuxtaK-142)
Plasmid#209013PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
Tags6xHis-msfGFPExpressionBacterialMutationaa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…Available SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(80 JuxtaK-421)
Plasmid#209008PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutationsDepositorInsertsyt1 (Syt1 Rat)
Tags6xHis-SUMOExpressionBacterialMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…Available SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP8B1(E234Q)-HA
Plasmid#209253PurposeMammalian expression of ATP8B1 mutant E234QDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pML45
Plasmid#206995PurposeContains gRNA targeting mouse Ptbp1 gene and Cas9 ORF.DepositorAvailable SinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC-cUnaG-LPETG
Plasmid#207635PurposeA plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.DepositorInsertphotocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
Tags6xHisExpressionBacterialMutationAmber stop codon at SC's critical lysinePromoterT7Available SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pnEA-NvG-HsNot3_656-753_T
Plasmid#147619PurposeBacterial Expression of HsNot3_656-753DepositorInsertHsNot3_656-753 (CNOT3 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only