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pCDB180

Plasmid

#80677

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged protein

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Type

Empty backbone

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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FKBP-CD28TMD-NTEVp(H75S)

Plasmid

#137828

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(H75S)

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Histidine 75 to Serine in NTEVp fragment

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAS

Plasmid

#140008

Purpose

Plasmid with 4xMmaPylT cassette to receive genes of interest for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

4xPylT (Mma) cassette

Expression

Mammalian

Mutation

U25C

Promoter

h7SK

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pACYC-yhaM-RFP

Plasmid

#207651

Purpose

Overexpression of native L-cysteine desulfidase and RFP in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

yhaM

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDR111_Bgal no NLS

Plasmid

#188397

Purpose

Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Tags

PhoD secretion peptide

Expression

Bacterial

Promoter

Phyper spank

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xHA-Reptin

Plasmid

#51636

Purpose

Mamamlian expression of 3xHA tagged reptin, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

reptin (RUVBL2 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-C.NR

Plasmid

#185672

Purpose

Expresses nuclease active eNme2-C.NR in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

eNme2-C.NR nuclease

Use

CRISPR

Expression

Mammalian

Mutation

eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLostfoX-Kan

Plasmid

#99865

Purpose

TfoX induction vector for chitin-pathway transformation (KanR)

Depositor

Mark Mandel

Article

Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111

Insert

tfoXVF

Use

V. fischeri suicide cloning vector, cmr, tcr

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-CyclinF

Plasmid

#32975

Depositor

Michele Pagano

Article

D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

Insert

CYCLIN F (CCNF Human)

Tags

Flag and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
pMSP1E2

Plasmid

#20063

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E2 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeats of he…

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-V2_hMSH2

Plasmid

#186155

Purpose

sgRNA targeting human MSH2 gene

Depositor

David McFadden

Article

Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

Insert

MSH2 (MSH2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-DDX3X

Plasmid

#70648

Purpose

Expresses WT DDX3X in mammalian cells under blasticidin selection.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

DDX3X (DDX3X Human)

Use

Retroviral

Tags

3x FLAG

Expression

Mammalian

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
mH2A1.2-CT-MYC

Plasmid

#45168

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy (MACROH2A1 Human)

Tags

HIS and MYC

Expression

Mammalian

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pET21 MBPx-SpyCatcher

Plasmid

#72327

Purpose

Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET21 MBPx-SpyCatcher

Tags

N-terminal His6 tag

Expression

Bacterial

Mutation

MBP contains A312V, I317V mutations and 172, 173,…

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dPlcR

Plasmid

#181832

Purpose

Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-TCF7L2

Plasmid

#232644

Purpose

transient expresses TCF7L2 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

Insert

TCF7L2 (TCF7L2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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gRNA-ura-HYB

Plasmid

#64330

Purpose

ura3 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of ura3 deletion gRNA cassette

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pSL1749 (pDonor, R*MmeI)

Plasmid

#160733

Purpose

Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST donor DNA

Expression

Bacterial

Promoter

N/A

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSprayerHE

Plasmid

#165150

Purpose

Sprayer plasmid for PROBER encoding BASU-GAL4 fusion (High Expression)

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Insert

BASU-GAL4

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMSP1E1

Plasmid

#20062

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeat of hel…

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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