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p128Tol2-Olactb:Cas9-t2A-GFP, 4xU6:sgRNA

Plasmid

#226834

Purpose

Expression of b-actin driven Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

Olactb and U6

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-nonstop

Plasmid

#226406

Purpose

Expresses EGFP without a stop codon that produces a GFP-tagged readthrough RQC substrate.

Depositor

Nico Dantuma

Article

Xu et al Commun Biol. 2024 Oct 8;7(1):1282. doi:

Insert

EGFP without stop codon

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_CloningVector_tagBFP

Plasmid

#163747

Purpose

Empty gRNA cloning vector, contains a tagBFP fluorescent marker for FACS sorting after transfection.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_CloningVector_miRFP670

Plasmid

#163748

Purpose

Empty gRNA cloning vector, contains a miRFP670 fluorescent marker for FACS sorting after transfection.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa50H9

Plasmid

#179628

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa50H9

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa3G5

Plasmid

#179629

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa3G5

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa30F3

Plasmid

#179620

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa30F3

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa32G3

Plasmid

#179621

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa32G3

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAb132A11

Plasmid

#179622

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAb132A11

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa45C8S

Plasmid

#179623

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa45C8S

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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HULAa2F4

Plasmid

#179624

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

HULAa2F4

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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EINA20F1

Plasmid

#179626

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

EINA20F1

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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EINA62G7

Plasmid

#179627

Purpose

Expresses heliorhodopsin gene in E.coli cells

Depositor

Oded Beja

Article

Chazan et al Environ Microbiol. 2022 Jan;24(1):110-12

Insert

EINA62G7

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAF146

Plasmid

#222377

Purpose

Express Tasmanian devil PDL1 protein (saha_PDL1_FL_DN11026_c0_g1_i1)

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

PDL1

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_W208L

Plasmid

#221486

Purpose

NKX2-1 W208L DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

W208L

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_Long

Plasmid

#221471

Purpose

Gateway cloning of NKX2-1 long isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_W208L

Plasmid

#221474

Purpose

Gateway cloning of NKX2-1 W208L DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

W208L

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_V205F

Plasmid

#221473

Purpose

Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

V205F

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_V205F

Plasmid

#221485

Purpose

NKX2-1 V205F DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

V205F

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-SFFV_NKX2-1

Plasmid

#221496

Purpose

NKX2-1 short isoform lentiviral overexpression using SFFV promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

SFFV

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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