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pCAGGS-AnkG ZU5ex-3xHA

Plasmid

#213731

Purpose

Mammalian cell expression vector for overexpression of AnkG ZU5ex isoform in motor neurons.

Depositor

Chaolin Zhang

Publication

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

AnkG ZU5ex (Ank3 Mouse)

Expression

Mammalian

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk19/kcnk12l

Plasmid

#209001

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Publication

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk19 (kcnk12l Zebrafish)

Use

Cloning

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(ERED198-201AAAA)-3xFLAG-pCDNA

Plasmid

#201965

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Publication

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

E198A, R199A,E200A, D201A

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pMX1463

Plasmid

#208805

Purpose

Expresses plasma-membrane-bound FRB-SBFP2 for rapid relocalization of cytoplasmic proteins tagged with FKBP upon addition of rapamycin

Depositor

Linda Wordeman

Publication

Wordeman et al Mol Biol Cell. 2016 Apr 15;27(8):1300-9.

Insert

FRB (CRP Human)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pETmsGFPSyt1(80 JuxtaK-142)

Plasmid

#209013

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

Depositor

Edwin Chapman

Publication

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis-msfGFP

Expression

Bacterial

Mutation

aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETSUMOSyt1C2AB(80 JuxtaK-421)

Plasmid

#209008

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations

Depositor

Edwin Chapman

Publication

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B1(E234Q)-HA

Plasmid

#209253

Purpose

Mammalian expression of ATP8B1 mutant E234Q

Depositor

Hye-Won Shin

Publication

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B1 (ATP8B1 Human)

Use

Retroviral

Tags

HA

Mutation

E234Q

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pML45

Plasmid

#206995

Purpose

Contains gRNA targeting mouse Ptbp1 gene and Cas9 ORF.

Depositor

Eugene Makeyev

Publication

Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

Insert

Ptbp1 gRNA (Ptbp1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSC-cUnaG-LPETG

Plasmid

#207635

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.

Depositor

Cole DeForest

Publication

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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p2888

Plasmid

#198146

Purpose

Epitope tagging of effector protein SseG

Depositor

Michael Hensel

Publication

Goser et al Nat Commun. 2023 Mar 4;14(1):1240. doi:

Insert

PsseA::sscB sseF::HA sseG::M45 (sseG Salmonella enterica serovar Typhimurium)

Tags

M45

Expression

Bacterial

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pnEA-NvG-HsNot3_656-753_T

Plasmid

#147619

Purpose

Bacterial Expression of HsNot3_656-753

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_656-753 (CNOT3 Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsNot3_589-753_T

Plasmid

#147620

Purpose

Bacterial Expression of HsNot3_589-753

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_589-753 (CNOT3 Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsNot3_656-753_T

Plasmid

#147621

Purpose

Bacterial Expression of HsNot3_656-753

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_656-753 (CNOT3 Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot2-del380-404-W507E_S

Plasmid

#147488

Purpose

Mammalian Expression of HsNot2-del380-404-W507E

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2-del380-404-W507E (CNOT2 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-HsNRF2 (NFE2L2)

Plasmid

#194302

Purpose

Multisite gateway entry clone for expression of human NRF2 (NFE2L2) with fusion tag at the N-terminus. Parton lab clone KRS

Depositor

Rob Parton

Publication

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

NFE2L2 (NFE2L2 Human)

Use

Multisite gateway entry vector

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-DrCavin1b

Plasmid

#194292

Purpose

Multisite gateway entry clone for expression of codon optimised zebrafish cavin1b with fusion tag at the N-terminus. Parton lab clone KXJ

Depositor

Rob Parton

Publication

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1b (cavin1b Zebrafish)

Use

Multisite gateway entry vector

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_GFP11-14-SEPT7

Plasmid

#180342

Purpose

mammalian expression of human SEPT7 fused to GFP11

Depositor

Manos Mavrakis

Publication

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA

Plasmid

#186407

Purpose

Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined gene of interest under control of an AttB3/B5 recombined reg. sequence.

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::RfA-ECFP

Plasmid

#186402

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-pSP172BSSPE-Swa1::venus-RfA

Plasmid

#186398

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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