Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 5,756 results for: PEP

Showing: 1021 - 1040 of 5756 results

Results per page:

Sort results by:

pLEX305-N-dTAG-CARD8-ZUC

Plasmid

#169990

Purpose

Mammalian expression of the dTAG-CARD8 ZUC (L162M-L537) fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1).

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

dTAG

Expression

Mammalian

Promoter

hPGK

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 CARD8 PAM

Plasmid

#169975

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 (no stop codon) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

PAM

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 CARD8 PAM L368G

Plasmid

#169978

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

PAM, L368G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 CARD8 PAM R394E

Plasmid

#169980

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

PAM, R394E

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 CARD8 PAM S297A

Plasmid

#169976

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

PAM, S297A

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 CARD8 FIIND E274R

Plasmid

#169967

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

E274R

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 CARD8 FIIND R394E

Plasmid

#169971

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

R394E

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 CARD8 FIIND F405G

Plasmid

#169973

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

F405G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 CARD8 PAM F370G

Plasmid

#169979

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

PAM, F370G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 CARD8 PAM F405G

Plasmid

#169981

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

PAM, F405G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8-FLAG R394E

Plasmid

#169954

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (R394E)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

R394E

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG L368G

Plasmid

#169959

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

L368G

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG R394E

Plasmid

#169961

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

R394E

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F405G

Plasmid

#170119

Purpose

Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed).

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Mutation

F405G

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG

Plasmid

#169958

Purpose

Mammalian expression of the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG and Ub

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8-FLAG L368G

Plasmid

#169952

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (L368G)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

L368G

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8-FLAG E274R

Plasmid

#169950

Purpose

Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E274R

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 LIC 6A CARD8-FLAG F370G

Plasmid

#169953

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (F370G)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

F370G

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS-rPALcc

Plasmid

#145804

Purpose

expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells

Depositor

Betty Eipper, Richard Mains

Article

Kolhekar et al Biochemistry. 2002 Oct 15;41(41):12384-9

Insert

rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)

Expression

Mammalian

Mutation

changed serine 767 to alanine to prevent glycosyl…

Promoter

CMV

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGS_N_Strep

Plasmid

#101651

Purpose

Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, Twin-Strep tag

Tags

Twin-Strep tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1021 - 1040 of 5756 results

Results per page:

Sort results by: