We narrowed to 11,805 results for: NSI;
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Plasmid#120956PurposeMoClo golden gate assembly CD part for sfGFP (Superfolder green fluorescent protein from DOI: 10.1038/nbt1172; slight variant on https://www.fpbase.org/protein/superfolder-gfp/)DepositorInsertsfGFP
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C13m
Plasmid#120960PurposeMoClo golden gate assembly CD part for Cerulean (modified CFP; variant of https://www.fpbase.org/protein/cerulean/) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertCerulean
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P30m
Plasmid#120942PurposeMoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed promoter, as used in DOI:10.1038/35002125). Please see Supplemental Documents for annotated Genbank file.DepositorInsertpL-lacO1
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pBP-P_cymR-PCymRC
Plasmid#204071PurposeType 0 promoter partDepositorInsertcymR repressor and PCymRC cumic acid-inducible promoter
ExpressionBacterialMutationNoneAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
C79m
Plasmid#120991PurposeMoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.DepositorInsertccdA antitoxin
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_vanR-PVanCC
Plasmid#204078PurposeType 0 promoter partDepositorInsertvanR repressor and PVanCC vanillic acid-inducible promoter
ExpressionBacterialMutationNoneAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTU2-a_Sv1KA_amilCP
Plasmid#204054PurposeType TU2-a backbone with kanamycin resistance, pSC101-var1 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.DepositorTypeEmpty backboneExpressionBacterialMutationNoneAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU2-a_Sv2KA_amilCP
Plasmid#204053PurposeType TU2-a backbone with kanamycin resistance, pSC101-var2 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.DepositorTypeEmpty backboneExpressionBacterialMutationNoneAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
V37m
Plasmid#121043PurposeMoClo golden gate assembly AE destination vector DVCK-AE-mScar (ColE1/kan resistance destination vector with constitutive mScarlet expressing for screening.DepositorInsertKan destination vector with ColE1 origin and mScarlet selection
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C27m
Plasmid#120965PurposeMoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.DepositorInserttetR (no deg tags)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C98m
Plasmid#121002PurposeMoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.DepositorInsertGp2 toxin
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C51m
Plasmid#120975PurposeMoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank fileDepositorInsertsfYFP (has BbsI site)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C33m
Plasmid#120968PurposeMoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.DepositorInsertAiiA degradase (Bennett, no ssra tag)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_nahR-PSalTTC
Plasmid#204074PurposeType 0 promoter partDepositorInsertnahR repressor and PSalTTC sodium salicylate-inducible promoter
ExpressionBacterialMutationNoneAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU2-a_Sv5KA_amilCP
Plasmid#204050PurposeType TU2-a backbone with kanamycin resistance, pSC101-var5 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.DepositorTypeEmpty backboneExpressionBacterialMutationNoneAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-T_BBa-B1006-U10
Plasmid#204122PurposeType 0 terminator partDepositorInsertBBa-B1006-U10 strong terminator
ExpressionBacterialMutationNoneAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU2-a_Sv3KA_amilCP
Plasmid#204052PurposeType TU2-a backbone with kanamycin resistance, pSC101-var3 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.DepositorTypeEmpty backboneExpressionBacterialMutationNoneAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU2-a_Sv6KA_amilCP
Plasmid#204049PurposeType TU2-a backbone with kanamycin resistance, pSC101-var6 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.DepositorTypeEmpty backboneExpressionBacterialMutationNoneAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU2-a_Sv7KA_amilCP
Plasmid#204048PurposeType TU2-a backbone with kanamycin resistance, pSC101-var7 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.DepositorTypeEmpty backboneExpressionBacterialMutationNoneAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_luxR-PLuxB
Plasmid#204073PurposeType 0 promoter partDepositorInsertluxR-PLuxB repressor and PLuxB 3OC6-AHL-inducible promoter
ExpressionBacterialMutationNoneAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTU2-a_Sv4KA_amilCP
Plasmid#204051PurposeType TU2-a backbone with kanamycin resistance, pSC101-var4 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.DepositorTypeEmpty backboneExpressionBacterialMutationNoneAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
T18m
Plasmid#121037PurposeMoClo golden gate assembly DE part for L3S2P21 (382x-strength synthetic terminator with BsaI cut site removed; see DOI: 10.1038/nmeth.2515).DepositorInsertmodified L3S2P21 (synthetic Voigt terminator)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
V35m
Plasmid#121042PurposeMoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.DepositorInsertKan destination vector with ColE1 origin and GFP selection
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U17m
Plasmid#121023PurposeMoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.DepositorInsertBujard RBS
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U19m
Plasmid#121024PurposeMoClo golden gate assembly BC part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertMG-apt
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C85m
Plasmid#120996PurposeMoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.DepositorInsertSigma X (ECF20_992)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C91m
Plasmid#120999PurposeMoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertsfCFP (Chen, Bennett)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C99m
Plasmid#121003PurposeMoClo golden gate assembly CD part for codon-optimized mScarlet-I (fast-folding mScarlet variant, codon-optimized for E. coli; see https://www.fpbase.org/protein/mscarlet-i/).DepositorInsertmScarlet-I codon optimized
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C21m
Plasmid#120962PurposeMoClo golden gate assembly CD part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertMG-apt
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3m
Plasmid#120935PurposeMoClo golden gate assembly AB part for J23150 (strength ~400x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertJ23150
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p4m
Plasmid#120936PurposeMoClo golden gate assembly AB part for J23151 (strength ~900x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertJ23151
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EcMscL
Plasmid#92418PurposeE. coli Mechanosensitive Channel of Large ConductanceDepositorInsertMscL (mscL E. coli)
Tags6x Histidine Tag and Thrombin Cleavage Site betwe…ExpressionBacterialPromoterT7 PromoterAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
C83m
Plasmid#120994PurposeMoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.DepositorInsertpir protein required for R6K ori
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U25m
Plasmid#121027PurposeMoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.DepositorInsertRiboJ + UTR1
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T16m
Plasmid#121035PurposeMoClo golden gate assembly DE part for L3S2P55 (256x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.DepositorInsertL3S2P55 (synthetic Voigt terminator)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T17m
Plasmid#121036PurposeMoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.DepositorInsertL3S2P11 (synthetic Voigt terminator)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_TALEsp1_PUPsp1-phlF_PPhlF
Plasmid#204080PurposeType 0 promoter partDepositorInsertTALEsp1-stabilized promoter driving phlF repressor; PPhlF DAPG-inducible promoter
ExpressionBacterialMutationNoneAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_lacI-Ptac-lacO
Plasmid#204072PurposeType 0 promoter partDepositorInsertlacI repressor and Ptac-lacO IPTG-inducible promoter
ExpressionBacterialMutationNoneAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
U24m
Plasmid#121026PurposeMoClo golden gate assembly BC part for RiboJ-BCD2 (low expression bi-cistronic RBS, engineered for context-independence, prefixed by self-cleaving RiboJ; see https://doi.org/10.1038/nmeth.2404).DepositorInsertRiboJ + BCD2
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only